Anti-Occludin Rabbit Polyclonal Antibody for WB, IF/ICC, IF-P, IHC-P, ELISA - Q16625

Core Product Specifications and Parameters

Biological Background: Occludin Function and Localization

• Occludin is a tight junction integral membrane protein encoded by the OCLN gene; alternative splicing produces multiple isoforms that contribute to junctional diversity and regulation (Keywords: Alternative splicing).
• It functions as a critical component of the paracellular permeability barrier, directly mediating cell-cell adhesion when expressed in cells lacking tight junctions.
• Subcellularly, occludin localizes to the cell membrane at tight junctions, interacting with the junctional complex to maintain epithelial and endothelial barrier integrity.
• Tissue distribution includes high expression in kidney and broad presence at tight junctions of both epithelial and endothelial cells; expression is notably absent in testis.
• Occludin contains transmembrane helices and a coiled-coil domain, hallmarks of tight junction proteins that facilitate membrane integration and protein-protein interactions (Keywords: Coiled coil, Transmembrane, Transmembrane helix).
• Post-translational modifications such as phosphorylation and disulfide bond formation are predicted to regulate its localization and barrier function (Keywords: Phosphoprotein, Disulfide bond).
• In addition to its junctional role, occludin acts as a coreceptor for hepatitis C virus (HCV) entry into hepatocytes, linking it to host-pathogen interactions.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 400–522 of human occludin; this region’s conservation across species may affect cross-reactivity, so validation in the relevant sample system is advised.
• For immunofluorescence or immunohistochemistry, consider that occludin localization to tight junctions may require optimized permeabilization and fixation protocols.
• Given the high kidney expression, using kidney lysate as a positive control in Western blot experiments may assist in verifying antibody specificity.
• For HCV entry studies, researchers should evaluate the antibody’s performance in hepatocyte models, as occludin’s coreceptor function may involve distinct conformational states.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated one-stop platform for IVD raw materials, offering streamlined access to validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target sourcing for tight junction and cell-barrier research. Whether you require diagnostic antibodies for permeability studies or specialized raw materials for HCV-related assays, our supply capabilities are designed to support manufacturers and laboratories from concept to clinic.