Anti-Occludin Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q16625

Core Product Specifications and Parameters

Biological Background: Occludin Function and Localization

• Occludin, encoded by the OCLN gene, is a ~59 kDa transmembrane tight junction protein (predicted molecular weight 59144 Da) containing coiled-coil domains and disulfide bonds.
• It plays a key role in the formation and regulation of tight junction paracellular permeability barriers and can induce adhesion when expressed in cells lacking tight junctions.
• Occludin acts as a coreceptor for hepatitis C virus (HCV) in hepatocytes, facilitating viral entry.
• The protein localizes at cell membranes, specifically at tight junctions, and is highly expressed in kidney; it is absent from testis.
• Occludin is classified as a phosphoprotein, and alternative splicing generates multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant protein/fragment, suggesting suitability for detecting endogenous Occludin across standard applications.
• For western blotting, expect a band around 59 kDa; validate in your sample system with appropriate controls.
• IHC-P and IF/ICC protocols should consider adequate fixation and antigen retrieval for tight junction targets.
• Cross-reactivity is reported for human, mouse, and rat samples; verify in your specific experimental context.
• ELISA may require optimization of coating conditions and antibody dilution for optimal signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

As a specialized IVD raw-material partner, CamelBio offers streamlined access to validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. This anti-Occludin monoclonal antibody supports research into tight junction biology and viral entry mechanisms, with CamelBio providing one-stop sourcing for rare target antibodies and customized material solutions. Our supply chain capabilities help diagnostic manufacturers and research labs bridge from concept to clinic with reliable raw materials.