Anti-OBSCN Polyclonal Antibody for WB, IF/ICC, IF-P, ELISA - Q5VST9

Core Product Specifications and Parameters

Biological Background: Obscurin Function and Localization

• Obscurin (gene OBSCN) is a giant scaffolding protein (~868 kDa) expressed in striated muscle, essential for myofibrillogenesis and sarcomere organization (PubMed:11448995, PubMed:16205939). Related references: PMID:11448995 PMID:16205939
• It localizes to both M-lines and Z-lines of sarcomeres, as well as the sarcolemma and nucleus, playing roles in structural integrity and signaling.
• Acts as a serine/threonine kinase that phosphorylates N-cadherin (CDH2) and the sodium/potassium-ATPase subunit ATP1B1 (By similarity).
• Binds specific phosphoinositides via its PH domain, with high affinity for PtdIns(3,4)P2 and PtdIns(4,5)P2, linking membrane dynamics to cytoskeletal architecture (PubMed:28826662). Related references: PMID:28826662
• Contains multiple immunoglobulin domains, fibronectin type III repeats, SH3 domains, and RhoGEF and MLCK-like kinase domains, indicating multifunctional scaffold and signaling capabilities.
• Alternative splicing produces distinct isoforms, including Obscurin-RhoGEF and Obscurin-MLCK, which likely have specialized tissue-specific roles.
• Keywords: muscle protein, kinase, lipid-binding, developmental protein, differentiation, disease variant – underscoring its importance in muscle development and disease.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a conserved region within the N-terminal portion of human OBSCN; cross-reactivity with mouse and rat suggests sequence homology in this domain.
• For Western blotting, account for the extremely large molecular weight (~868 kDa) by using appropriate gel systems (e.g., low-percentage/gradient gels and extended transfer conditions) and a high-molecular-weight marker.
• In immunofluorescence/immunocytochemistry, typical staining is observed at M-lines and Z-lines; co-localization with established sarcomeric markers (e.g., α-actinin) is recommended for validation.
• ELISA may be performed using the recombinant immunogen as a control; optimization of coating and detection conditions is advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive portfolio of IVD-grade antibodies and ancillary reagents tailored for muscle disease biomarker development and sarcomere protein research. With validated polyclonal antibodies like this anti-OBSCN, alongside custom antibody production, bulk antigen supply, and technical consultation, CamelBio accelerates your assay development from concept to clinic. Our expertise in rare target raw-material sourcing ensures you have the critical tools needed for striated muscle and myofibrillogenesis studies.