Anti-OAZ1 Rabbit Polyclonal Antibody for WB, ELISA - P54368

Core Product Specifications and Parameters

Biological Background: OAZ1 Function and Localization

• OAZ1 (Ornithine decarboxylase antizyme 1), also known as AZ1, AZI, OAZ, is a key negative regulator of polyamine biosynthesis and uptake.
• It directly binds to ODC monomers, preventing the formation of the catalytically active ODC homodimer, and targets ODC monomers for ubiquitin-independent proteasomal degradation, partly by exposing a cryptic proteasome-interacting surface on ODC.
  Related references:
  PMID:17900240
  PMID:26305948
  PMID:26443277
• OAZ1 stabilizes AZIN2 (antizyme inhibitor 2) by interfering with its ubiquitination, thereby fine-tuning polyamine homeostasis.
  Related references:
  PMID:17900240
• The protein inhibits cellular polyamine uptake by inactivating the polyamine transporter, providing an additional layer of control over intracellular polyamine levels.
• OAZ1 participates in a SMAD1/OAZ1/PSMB4 complex that mediates the degradation of the transcriptional repressor SNIP1.
• It is involved in the translocation of AZIN2 from the ER-Golgi intermediate compartment (ERGIC) to the cytosol.
  Related references:
  PMID:12097147
• Expression of OAZ1 requires programmed ribosomal frameshifting, a unique regulatory mechanism. The protein has been structurally characterized, with a 3D structure available.

Experimental Guidance and Technical Tips

• The immunogen used corresponds to the N-terminal region (aa 1–68) of human OAZ1; consider this when designing epitope‑tagged constructs or competition assays.
• For Western blot, a band near 25 kDa is expected. Validate antibody specificity in the relevant sample system, as distinct isoforms or post‑translational modifications may affect migration.
• This antibody has demonstrated cross‑reactivity with mouse and rat OAZ1, as indicated; verify suitability in your experimental context via appropriate controls.
• For ELISA, titrate the antibody against the recombinant immunogen or native target to determine optimal coating and detection conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive partner for IVD raw material sourcing, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. We support your polyamine metabolism research and diagnostic development efforts with a streamlined supply chain linked to this OAZ1 polyclonal antibody. Our team assists from concept to clinic, including customized rare‑target reagent sourcing and technical guidance.