Anti-OASL Rabbit Polyclonal Antibody for WB, ELISA - Q15646

Core Product Specifications and Parameters

Biological Background: OASL Function and Localization

• OASL (2'-5'-oligoadenylate synthase-like protein), also known as p59 OASL or TRIP14, is a member of the 2'-5'-oligoadenylate synthetase (OAS) family but lacks 2'-5'-OAS catalytic activity.
• It binds double-stranded RNA and exerts antiviral activity against encephalomyocarditis virus (EMCV) and hepatitis C virus (HCV) through an RNase L-independent pathway.
• OASL localizes primarily to the nucleus, nucleolus, and cytoplasm, reflecting its multifaceted role in innate immunity.
• The protein is widely expressed in human tissues, with highest levels in primary blood leukocytes, hematopoietic tissues, colon, stomach, and testes.
• Multiple OASL isoforms exist due to alternative splicing, with predicted molecular weights of 29 kDa, 43 kDa, and 59 kDa; the canonical isoform is 59 kDa.
• Post-translational modifications include acetylation, and the protein features a repeat domain structure conducive to RNA binding.

Experimental Guidance and Technical Tips

• The immunogen encompasses amino acids 1–350 of the human OASL protein (NP_003724.1), targeting the N-terminal region common to isoforms.
• In western blotting, multiple bands corresponding to the 29 kDa, 43 kDa, and 59 kDa isoforms may be observed, depending on the tissue or cell type.
• For ELISA applications, consider coating with recombinant OASL or cell lysates from OASL-expressing lines such as leukocytes or colon-derived cells.
• Ensure validation in the appropriate species (human, mouse) and relevant biological samples where OASL expression is highest.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research labs with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, monoclonal and polyclonal antibodies, and bulk ancillary reagents. This anti-OASL rabbit polyclonal antibody is well-suited for researchers studying antiviral innate immunity and interferon signaling pathways. For specialized needs such as rare target sourcing or custom raw-material development, CamelBio offers tailored supply solutions to accelerate your project from concept to clinic.