Anti-OAS3 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q9Y6K5

Core Product Specifications and Parameters

Biological Background: OAS3 Function and Localization

• OAS3 (2'-5'-oligoadenylate synthase 3), also known as p100 OAS, is an interferon-induced enzyme critical for innate antiviral immunity.
• OAS3 catalyzes the dimerization of ATP into 2'-5'-diadenylate (2-5A), which then activates RNase L to degrade viral and cellular RNA, inhibiting protein synthesis and viral replication.
• The protein exhibits antiviral activity against Chikungunya virus (CHIKV), Dengue virus, Sindbis virus (SINV), and Semliki forest virus (SFV), and can function via both RNase L-dependent and independent pathways.
• OAS3 is the largest member of the OAS gene family and contains multiple OAS domains, contributing to its unique enzymatic properties.
• Subcellular localization includes both the cytoplasm and nucleus, where it may participate in distinct antiviral mechanisms.
• Key post-translational modifications include acetylation and phosphorylation, as indicated by proteomics data.
• OAS3 is highly expressed in placental trophoblasts, suggesting a role in protecting the fetus from viral infections.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 320-620 of human OAS3, a region that may be conserved across species; cross-reactivity with mouse and rat has been reported.
• For Western blot, a band at approximately 121 kDa is expected; use appropriate molecular weight markers and consider the potential for post-translational modification shifts.
• For immunohistochemistry (IHC-P), placental tissue sections are a recommended positive control due to high OAS3 expression in trophoblasts.
• In immunofluorescence (IF/ICC), both cytoplasmic and nuclear staining may be observed; fixation and permeabilization protocols should be optimized accordingly.
• ELISA performance should be validated using recombinant OAS3 or cell lysate standards.

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CamelBio offers diagnostic manufacturers and researchers a streamlined pathway to high-quality IVD raw materials, including validated polyclonal antibodies like this OAS3 rabbit pAb for innate immunity and antiviral studies. Our comprehensive portfolio covers optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and sourcing for rare targets, supporting your assay development from concept to clinic.