Anti-OAS1 Rabbit Polyclonal Antibody for WB and ELISA - P00973

Core Product Specifications and Parameters

Biological Background: OAS1 Function and Localization

• OAS1 encodes 2'-5'-oligoadenylate synthase 1, existing as p46 and p42 isoforms through alternative splicing, and is a member of the OAS family of interferon-induced proteins.
• It acts as a critical innate immune sensor and antiviral enzyme, activated by double-stranded RNA (dsRNA) to catalyze 2'-5'-oligoadenylate (2-5A) synthesis from ATP. Related references: PMID:34581622
• The 2-5A molecules bind to and dimerize latent RNase L, triggering its activation and leading to widespread RNA degradation, thereby inhibiting protein synthesis and blocking viral replication. Related references: PMID:34145065, PMID:34581622, PMID:40010341
• OAS1 contributes to intercellular immune communication that limits the local dissemination of RNA viruses and exerts tumor-suppressive functions. Related references: PMID:40010341
• C-terminal prenylation directs OAS1 to membranous replication organelles in SARS-CoV-2-infected cells, where it specifically binds conserved stem-loop structures in the viral 5'-UTR, activating RNase L and potently suppressing viral replication. Related references: PMID:34581622
• Subcellular localization studies reveal OAS1 in the cytoplasm, mitochondria, nucleus, microsomes, and endoplasmic reticulum, with a fraction also secreted.
• OAS1 is highly expressed in lung tissue, and its secreted form mediates antiviral effects against viruses such as VSV, HSV-2, and EMCV via RNase L-independent pathways.
• Key structural and functional features include ATP-binding, nucleotide-binding, prenylation, and participation in host-virus interactions and innate immunity.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide within the N-terminal region (aa 1-100) of human OAS1. This polyclonal antibody may recognize both p46 and p42 isoforms, but consider epitope accessibility differences between native and denaturing conditions. Validate isoform detection in your model system.
• For Western blot, the predicted molecular weight is approximately 46 kDa; however, alternative splicing or post-translational modifications may result in multiple bands. Include appropriate positive and negative controls to confirm specificity.
• In ELISA, the polyclonal nature of this antibody can provide high sensitivity. Consider testing it as either capture or detection reagent, and optimize pairing with a monoclonal antibody for sandwich ELISA formats targeting the 2-5A pathway.
• Reported cross-reactivity with mouse and rat suggests potential utility in rodent studies, but confirm reactivity and performance in the relevant sample matrix before use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted partner for IVD raw-material sourcing, offering a comprehensive portfolio that includes validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents to power your innate immunity and antiviral research. Whether you need this OAS1 antibody for diagnostic assay development or require rare-target sourcing for the 2-5A pathway, our supply capabilities help you move seamlessly from concept to clinic.