Anti-OAF Polyclonal Antibody for WB, ELISA - Q86UD1

Core Product Specifications and Parameters

Biological Background: OAF Function and Localization

• OAF (Out at first protein homolog) is a human protein encoded by the OAF gene, also referred to as HCV NS5A-transactivated protein 13 target protein 2 (NS5ATP13TP2).
• The UniProt entry Q86UD1 is a reviewed Swiss-Prot record and part of the Reference proteome, indicating high-quality annotation.
• It carries the keyword "Signal," indicating the presence of a signal peptide and predicted secretion from the cell.
• The calculated molecular weight of the precursor protein is 30,688 Da (UniProt); the mature protein, after cleavage of the signal peptide, would have a lower mass.
• This antibody was generated against a recombinant fragment spanning amino acids 28-150 (NP_848602.1), located after the predicted signal sequence, suggesting it recognizes the secreted or processed form.
• No detailed functional annotation is currently available in UniProt; the protein has been identified in large-scale proteomics experiments (keyword: Proteomics identification).

Experimental Guidance and Technical Tips

• For Western blot (WB), consider using a reduced denaturing SDS-PAGE gel with a protein ladder covering the 25–35 kDa range. The predicted size of the OAF precursor is ~31 kDa; the mature form may appear slightly lower.
• The polyclonal nature of the antibody may yield robust signal in ELISA; validate with the appropriate recombinant protein or cell lysates as controls.
• Due to reported cross-reactivity with mouse and rat OAF, this antibody can be tested on samples derived from these species, though validation in the relevant system is recommended.
• If non-specific background is observed, consider optimizing blocking conditions or performing a pre-adsorption step using the immunogen fragment.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material partner, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and customized procurement services. This OAF polyclonal antibody is positioned to support research in viral pathogenesis and proteomics. Beyond this product, CamelBio leverages an extensive supplier network to source difficult-to-find targets and provides technical support throughout assay development, ensuring a streamlined path from research to diagnostic application.