Anti-NYX Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q9GZU5

Core Product Specifications and Parameters

Biological Background: NYX Function and Localization

• Nyctalopin (NYX) is a secreted proteoglycan with a predicted molecular weight of approximately 51.5 kDa (UniProt: Q9GZU5).
• The protein is a member of the small leucine-rich repeat proteoglycan (SLRP) family, characterized by leucine-rich repeat (LRR) motifs.
• NYX plays a critical role in vision by mediating light-induced depolarization of retinal ON-bipolar cells, likely acting as a scaffold for the proteins TRPM1 and GRM6.
• It is essential for the proper trafficking of TRPM1 to the dendritic tips of ON-bipolar cells, thereby facilitating signal transduction at the photoreceptor-bipolar synapse.
• Subcellularly, NYX is localized to the extracellular matrix, dendrites, and postsynaptic regions, and is secreted into the extracellular space.
• Tissue expression is prominent in the retina (inner segment of photoreceptors, outer and inner nuclear layers, ganglion cell layer) and kidney, with lower levels in brain, testis, and muscle.
• Mutations in the NYX gene are associated with congenital stationary night blindness (CSNB), an inherited retinal disorder characterized by impaired night vision.
• Post-translational modifications include glycosylation and proteoglycan formation, consistent with its extracellular matrix function.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a fragment within the C-terminal region of human NYX (aa 282-481), which contains several leucine-rich repeats. This design may confer target specificity; however, cross-reactivity should be validated in the relevant sample system.
• For Western blotting, a band around 52 kDa is expected. It is advisable to include positive controls (e.g., retinal lysates) and use appropriate blocking and washing conditions to minimize background.
• In immunohistochemistry (paraffin sections), antigen retrieval may be required for optimal detection. Test on human or mouse retinal tissue sections, where NYX is expressed in the inner segments and nuclear layers.
• For ELISA applications, consider using the recombinant protein as a coating antigen and titrate both coating and detection antibodies to achieve linear dose-response.
• Due to the polyclonal nature of the antibody, it may detect multiple epitopes; therefore, specificity should be confirmed using knockout or knockdown models if available.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides end-to-end sourcing of IVD raw materials, from validated antibody pairs and optimized monoclonal/polyclonal antibodies to bulk ancillary reagents. This anti-NYX antibody supports investigations into retinal biology and vision-related disorders. For rare targets or customized needs, our team facilitates tailored sourcing and consulting services to accelerate your diagnostic development pipeline.