Anti-NY-ESO-1 Rabbit Monoclonal Antibody for WB, ELISA - P78358

Core Product Specifications and Parameters

Biological Background: NY-ESO-1 Function and Localization

• Cancer/testis antigen 1 (NY-ESO-1), encoded by the CTAG1A and CTAG1B genes, is a highly immunogenic protein identified in various cancers and normal testis.
• It belongs to the cancer/testis antigen family, exhibiting restricted expression in immune-privileged tissues and frequent aberrant re-expression in tumors, making it a key target for cancer immunotherapy.
• NY-ESO-1 is predominantly localized in the cytoplasm, as indicated by subcellular location data and consistent with its role as an intracellular cancer-testis antigen.
• Tissue-specific expression includes adult testis (spermatogonia and primary spermatocytes), ovary, uterine myometrium, and a wide range of cancers; fetal testis expression is observed from 18 weeks until birth.
• The protein undergoes alternative splicing, contributing to isoform diversity, and its 3D structure has been resolved, facilitating structural biology and antibody engineering studies.
• With a calculated molecular weight of ~18 kDa, NY-ESO-1 is a small protein that serves as an informative biomarker for diagnostic and prognostic applications in oncology.

Experimental Guidance and Technical Tips

• The antibody was raised against a synthetic peptide corresponding to the N-terminal region (aa 1-100) of human NY-ESO-1 and detects endogenous protein at ~18 kDa in western blotting.
• For WB, a specific band at 18 kDa is expected; consider validating the antibody's sensitivity and specificity in your target sample system, as expression levels can vary widely across tissues and tumor types.
• In ELISA, pair this monoclonal antibody with an appropriate detection antibody recognizing a distinct epitope; optimize coating concentrations and blocking conditions to achieve robust signal-to-noise ratios.
• Cross-reactivity is reported only with human; performance in other species should be experimentally confirmed if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research teams with comprehensive IVD raw-material supply. This NY-ESO-1 Rabbit mAb is part of our portfolio of validated monoclonal antibodies for cancer biomarker detection. Beyond single antibodies, we offer matched antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets—ensuring a reliable supply chain from concept to clinical application.