Anti-NY-ESO-1 Polyclonal Antibody for WB, IHC-P, ELISA - P78358

Core Product Specifications and Parameters

Biological Background: NY-ESO-1 Function and Localization

• Cancer/testis antigen 1 (NY-ESO-1, also designated CTAG1, CT6.1, or LAGE-2) is a 180-amino acid protein with a calculated molecular weight of approximately 18 kDa (UniProt entry P78358).
• The NY-ESO-1 antigen is encoded by the CTAG1A and CTAG1B genes, which produce alternatively spliced transcripts (UniProt keyword "Alternative splicing").
• NY-ESO-1 is classified as a cancer/testis antigen; its normal expression is restricted to testis and ovary, while it is aberrantly expressed in a wide variety of cancers.
• Subcellularly, NY-ESO-1 localizes to the cytoplasm (UniProt SUBCELLULAR LOCATION: Cytoplasm).
• In the adult testis, strong expression is detected in spermatogonia and primary spermatocytes, but not in post-meiotic cells or somatic cells (at protein level).
• Fetal testis expression begins at 18 weeks and persists until birth; it is also detected in uterine myometrium.
• No molecular function has been annotated for NY-ESO-1 in UniProt, but its restricted expression pattern suggests a role in germ cell biology and cancer immunology.

Experimental Guidance and Technical Tips

• This antibody was generated against a synthetic peptide spanning the N-terminal region (amino acids 1–100) of human NY-ESO-1; it is polyclonal and may recognize epitopes within this region.
• For Western blotting, a band of approximately 18 kDa is expected; consider using total cell lysates from NY-ESO-1-positive cancer cell lines or testis tissue as positive controls, and NY-ESO-1-negative cell lines as negative controls.
• In immunohistochemistry (IHC-P), antigen retrieval optimization may be necessary; nuclear/cytoplasmic staining patterns may vary depending on the sample; include isotype controls.
• For ELISA, consider direct or sandwich formats; the peptide immunogen may be used as a positive control to validate assay specificity.
• Because endogenous NY-ESO-1 expression is restricted to germ cells and cancers, ensure proper tissue-specific and cancer-type controls are included in each experiment.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research institutes with integrated access to IVD raw materials, including validated polyclonal and monoclonal antibodies, antibody pairs, and ancillary reagents. For NY-ESO-1, a key cancer/testis antigen target, CamelBio can source bulk-quantity antibodies optimized for ELISA, immunohistochemistry, and blotting applications, as well as assist with custom reagent development and rare-target procurement to accelerate your assay development pipeline.