Anti-NXT2 Polyclonal Antibody for WB, IF/ICC, ELISA - Q9NPJ8

Core Product Specifications and Parameters

Biological Background: NXT2 Function and Localization

• NXT2 is NTF2‑related export protein 2, also referred to as protein p15‑2. It is encoded by the NXT2 gene in humans.
• NXT2 functions as a regulator of nuclear export for proteins containing a nuclear export signal (NES). It also participates in mRNA nuclear export.
• The protein localizes to both the nucleus and the cytoplasm, consistent with its role in nucleocytoplasmic shuttling.
• Alternative splicing of the NXT2 transcript gives rise to multiple isoforms, potentially expanding functional diversity.
• UniProt keywords include “Alternative splicing”, “Cytoplasm”, “mRNA transport”, “Nucleus”, “Protein transport”, and “Transport”, underscoring its central role in intracellular trafficking.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 110–180 of human NXT2; ensure that your target region is present in the tested samples or isoforms.
• In Western blotting, expect a band near 16 kDa; verify specificity using positive and negative control lysates from human, mouse, or rat cells.
• For immunofluorescence, consider co‑staining with nuclear markers (e.g., DAPI) and cytoplasmic markers to confirm NXT2 localization.
• Performance in ELISA may depend on coating buffer and antigen presentation; optimization is recommended.
• As with all polyclonal antibodies, validate lot‑to‑lot consistency and perform necessary blocking steps to reduce non‑specific binding.

CamelBio: Your One‑Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, laboratories, and research institutes with a comprehensive pipeline of IVD raw materials, technical services, and consulting. For NXT2‑related studies, we can supply validated antibody pairs, optimized polyclonal antibodies, bulk ancillary reagents, and assist with sourcing rare‑target raw materials. Contact us to simplify your procurement and accelerate nuclear transport research.