Anti-NXF1 Rabbit Polyclonal Antibody for WB and ELISA - Q9UBU9

Core Product Specifications and Parameters

Biological Background: Nuclear RNA Export Factor 1 (NXF1) Function and Localization

• NXF1, also known as TAP (Tip-associated protein) or MEX67, is a nuclear RNA export factor that mediates the nuclear export of mRNA.
• Forms a heterodimer with NXT1 and participates in the export of mRNA, including HSP70 mRNA, through interactions with ALYREF/THOC4 and THOC5 of the TREX complex. Also facilitates nuclear export of m6A-containing mRNAs by bridging SRSF3 and YTHDC1.
  Related references:
  PMID:10924507 PMID:18364396 PMID:19165146 PMID:9660949 PMID:28984244
• Localizes to multiple subcellular compartments including the nucleus, nucleoplasm, nuclear speckles, nuclear pore complex, and cytoplasm; can also be found in stress granules.
• Post-translational modifications include acetylation, methylation, nitration, and phosphorylation, which may regulate its activity or interactions.
• Ubiquitously expressed across tissues, reflecting its fundamental role in mRNA export.
• Contains leucine-rich repeats and RNA-binding domains, and undergoes alternative splicing, producing isoforms with potentially distinct functions.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to residues 200-300 of human NXF1. This internal region may yield antibodies suitable for detecting both full-length and certain truncated isoforms.
• For Western blot, a band around 70 kDa is expected; consider using reducing conditions and appropriate protein loading controls.
• The antibody is reported to react with human, mouse, and rat; verify cross-reactivity in your species of interest if necessary.
• For ELISA, optimize coating antigen and antibody dilutions; peptide-based antibodies may require care in epitope presentation.
• Given the ubiquitous expression of NXF1, endogenous levels can be detected in many cell lines, but signal intensity may vary; include positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive range of IVD raw materials, from validated antibody pairs and optimized polyclonal/monoclonal antibodies to ancillary reagents, supporting your mRNA export and nuclear transport research. As a one-stop sourcing partner, we streamline procurement for diagnostic manufacturers and laboratories, offering reliable access to rare-target raw materials to accelerate your projects from concept to clinic.