Anti-NXF1 Rabbit Monoclonal Antibody for WB, IP, ELISA - Q9UBU9

Core Product Specifications and Parameters

Biological Background: NXF1 Function and Localization

• NXF1 (Nuclear RNA export factor 1, also known as TAP) is a multi-domain mRNA export receptor that plays a central role in nucleocytoplasmic transport of mRNAs.
• It is the key component of the TAP/NXF1 pathway, mediating nuclear export of mRNA species bearing retroviral constitutive transport elements (CTE). Related references: PMID:10924507
• The NXF1-NXT1 heterodimer cooperates with TREX complex components ALYREF/THOC4 and THOC5 to export HSP70 mRNA, with ALYREF/THOC4-bound mRNA being transferred to the heterodimer for transport. Related references: PMID:18364396 PMID:19165146 PMID:9660949
• NXF1 also participates in nuclear export of m6A-containing mRNAs via interaction with SRSF3 and YTHDC1, facilitating mRNA nuclear export. Related references: PMID:28984244
• Subcellularly, NXF1 localizes predominantly to the nucleus (nucleoplasm, nuclear speckles, nuclear pore complex, nuclear envelope) and shuttles to the cytoplasm and stress granules.
• It is ubiquitously expressed across human tissues, reflecting its fundamental role in mRNA metabolism.
• Post-translational modifications including acetylation, methylation, phosphorylation, and nitration regulate its function, and it is implicated in host-virus interactions.

Experimental Guidance and Technical Tips

• The immunogen encompasses N-terminal residues 1-92, providing a robust epitope; confirm specificity using knockdown or knockout controls in your target system.
• For WB, standard reducing SDS-PAGE conditions with 70 kDa protein weight should be used; consider nuclear/cytoplasmic fractionation to enrich NXF1 signal.
• In IP, optimize antibody-to-lysate ratios and include appropriate isotype controls; NXF1’s association with RNA and protein partners may require RNase treatment for clean detection.
• ELISA protocols should employ recombinantly expressed NXF1 or cell lysates; validate with serial dilutions to ensure linearity.
• Since cross-reactivity is only tested for human, reactivities with other species are not guaranteed; if using non-human samples, verify cross-reactivity experimentally.
• Always titrate the antibody in your specific experimental setup to determine optimal working concentrations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated monoclonal antibodies like this anti-NXF1 antibody, optimized for applications such as mRNA export research and viral host interaction studies. As an end-to-end sourcing partner, we provide bulk reagent supplies, antibody pair development, and rare target procurement to support diagnostic manufacturers and research laboratories from concept to clinic.