Anti-NUTF2 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P61970

Core Product Specifications and Parameters

Biological Background: NUTF2 Function and Localization

• NUTF2 (Nuclear Transport Factor 2), also designated NTF2 and placental protein 15 (PP15), is a 14 kDa protein belonging to the NTF2 family and plays a key role in nucleocytoplasmic transport.
• It specifically mediates the import of GDP-bound RAN from the cytoplasm into the nucleus, a critical step that enables RAN to function in cargo receptor-mediated transport across the nuclear envelope.
• NUTF2 directly interacts with GDP-bound RAN in the cytosol and guides it to the nuclear pore complex via associations with nucleoporins, thereby promoting nuclear translocation.
• Subcellularly, NUTF2 localizes to the cytoplasm, nuclear pore complex, and both inner and outer nuclear membranes, consistent with its shuttling function.
• Post-translational modifications, including acetylation, and its involvement in mRNA and protein transport are documented in curated databases, highlighting its functional versatility.

Experimental Guidance and Technical Tips

• The immunogen is a full-length recombinant human NUTF2 protein (aa 1-127), so the resulting polyclonal antibody is expected to recognize multiple epitopes, conferring high sensitivity.
• For Western blotting, NUTF2 has a predicted molecular weight of ~14 kDa. Small proteins may require optimized transfer conditions (e.g., lower methanol concentration or shorter transfer time) to ensure efficient membrane binding.
• In immunofluorescence/ICC, the antibody should detect endogenous NUTF2 at the nuclear envelope and in the cytoplasm; permeabilization with Triton X-100 or digitonin may be needed to access nuclear and pore-complex pools.
• Cross-reactivity with rat has been demonstrated by the supplier, making this antibody suitable for rodent model studies; however, as with any new application, we recommend validation in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves diagnostic manufacturers and research laboratories as a comprehensive IVD raw-material sourcing partner, providing validated antibody pairs, bulk ancillary reagents, and support for rare target procurement. This NUTF2 antibody supports studies in nucleocytoplasmic transport and cargo receptor pathways. Whether you need optimized monoclonal/polyclonal antibodies, recombinant proteins, or custom services, CamelBio bridges the gap from concept to clinic with reliable supply solutions.