Anti-NUSAP1 Polyclonal Antibody for WB and ELISA - Q9BXS6

Core Product Specifications and Parameters

Biological Background: NUSAP1 Function and Localization

• NUSAP1 (Nucleolar and spindle-associated protein 1) is a microtubule-associated protein (MAP) that bundles and stabilizes microtubules, playing a critical role in mitotic spindle organization.
• It associates with chromosomes and facilitates the proper assembly and function of the mitotic spindle, ensuring accurate chromosome segregation during cell division.
• The protein localizes to multiple subcellular compartments, including the nucleolus, cytoplasm, cytoskeleton, spindle, and chromosomes, reflecting its dynamic role in mitosis.
• NUSAP1 is subject to alternative splicing, producing multiple isoforms that may have distinct functions or localizations.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitin-like (Ubl) conjugation, which likely regulate its activity and stability.
• It contains a coiled-coil domain and possesses DNA-binding capability, suggesting additional roles in chromatin or DNA-related processes.
• NUSAP1 is implicated in cell cycle progression and is often upregulated in proliferating cells, making it a potential biomarker for cell proliferation and cancer.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-61) of human NUSAP1; consider using this antibody in western blot (WB) and ELISA applications as indicated.
• Due to the recombinant nature of the immunogen, validate specificity for endogenous NUSAP1 in your target species (human and mouse).
• NUSAP1 undergoes alternative splicing; be aware of potential multiple bands in WB that correspond to different isoforms.
• For cell cycle studies, synchronize cells to enrich for mitotic populations where NUSAP1 expression and localization are most prominent.
• Include appropriate controls (e.g., lysates from cells with known NUSAP1 expression levels) to confirm antibody performance in your experimental setup.
• As a polyclonal antibody, it may offer robust signal in ELISA; titrate carefully to optimize signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a comprehensive one-stop platform for sourcing high-quality IVD raw materials. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and support for rare target sourcing. For researchers focused on NUSAP1 and mitosis-related targets, we offer reliable antibodies and technical support to accelerate your assay development and biomarker research. Partner with CamelBio to streamline your procurement from concept to clinic.