Anti-NUR77 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P22736

Core Product Specifications and Parameters

Biological Background: NUR77 Function and Localization

• Orphan nuclear receptor encoded by the NR4A1 gene, also known as TR3, NGFIB, NAK-1, and HMR.
• Binds the NGFI-B response element (NBRE) 5'-AAAGGTCA-3' to regulate transcription of target genes involved in apoptosis, cell proliferation, and metabolism. Related references: PMID:18690216 PMID:8121493 PMID:9315652
• Plays a role in energy homeostasis by sequestering STK11 in the nucleus, thereby attenuating cytoplasmic AMPK activation. Related references: PMID:22983157
• Modulates the inflammatory response in macrophages via metabolic reprogramming and inhibits NF-kappa-B signaling by binding to low-affinity κB sites, such as at the IL2 promoter. Related references: PMID:15466594
• Promotes non-canonical NLRP3 inflammasome activation upon detection of bacterial lipopolysaccharide and mitochondrial DNA during pyroptosis.
• Shuttles between nucleus, cytoplasm, and mitochondria; subcellular localization influences its diverse functions.
• Expressed in fetal muscle and adult liver, brain, and thyroid.
• Post‑translational modifications include acetylation and phosphorylation; contains a zinc‑finger DNA‑binding domain.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 300–400; for WB, a band at approximately 64 kDa is expected. Validate with positive controls such as HeLa or liver lysates.
• For IHC-P, optimize antigen retrieval conditions and antibody dilution. Cross‑reactivity with human and mouse tissues is reported.
• In ELISA applications, the peptide immunogen suggests suitability for screening; however, empirical titration and validation in the relevant sample matrix are recommended.
• Consider endogenous expression levels and stimulation conditions (e.g., serum starvation, inflammatory induction) to maximize detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with one‑stop access to a trusted portfolio of IVD raw materials, technical services, and consulting—from concept to clinic. For nuclear receptor targets like NUR77, we supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare‑target raw‑material sourcing to accelerate your assay development and clinical translation.