Anti-NUR77 (KD Validated) Rabbit Monoclonal Antibody for WB and ELISA - P22736

Core Product Specifications and Parameters

Biological Background: NUR77 Function and Localization

• Nuclear receptor subfamily 4 group A member 1 (NUR77, also known as NR4A1, NAK1, TR3, NGFIB) is an orphan nuclear receptor that binds the NGFI-B response element (NBRE) 5'-AAAGGTCA-3' and can interact with 9-cis-retinoic acid outside its ligand-binding domain. Related references: PMID:18690216 PMID:8121493 PMID:9315652
• NUR77 participates in energy homeostasis by sequestering STK11 in the nucleus, attenuating cytoplasmic AMPK activation, and regulates metabolic adaptations during macrophage inflammation, including repression of citric acid cycle genes. Related references: PMID:22983157
• It inhibits NF-kappa-B signaling by binding low-affinity NF-kappa-B sites, such as at the IL2 promoter, and may act with NR4A2 in liver regeneration. Related references: PMID:15466594
• In the cytosol, upon detecting bacterial LPS and mitochondrial DNA released during pyroptosis, NUR77 promotes non-canonical NLRP3 inflammasome activation by stimulating NLRP3-NEK7 association.
• Subcellular localization includes nucleus, cytoplasm, cytosol, and mitochondrion, supporting both transcriptional and cytoplasmic regulatory functions.
• Tissue expression is observed in fetal muscle, adult liver, brain, and thyroid.
• Post-translational modifications include phosphorylation and acetylation; it contains zinc-finger domains essential for DNA binding and receptor activity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 250-350 of human NUR77, a region that may influence epitope accessibility; consider this when designing experiments.
• Validated for Western blot (WB) and ELISA; when using other applications, additional optimization may be necessary.
• Cross-reactivity with human and mouse has been confirmed; verify reactivity in your specific sample context.
• For best results, follow standard protocols for rabbit monoclonal antibodies and validate in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with a one-stop sourcing platform for high-quality IVD raw materials, including this anti-NUR77 monoclonal antibody. Our portfolio covers nuclear receptor targets and a broad range of immunoassay reagents. For bulk orders, validated antibody pairs, or customized raw-material sourcing, contact CamelBio as your trusted procurement partner.