Anti-NUP98 Rabbit Monoclonal Antibody for WB - P52948

Core Product Specifications and Parameters

Biological Background: NUP98 Function and Localization

• NUP98 is a component of the nuclear pore complex (NPC) that plays a critical role in NPC assembly and maintenance.
• Together with NUP96, it mediates bidirectional transport across the NPC and anchors NUP153 and TPR to the complex. Related references: PMID:33097660
• In collaboration with DHX9, NUP98 regulates transcription and alternative splicing of specific genes and localizes DHX9 to intranuclear GLFG bodies. Related references: PMID:28221134
• The protein undergoes autocatalytic cleavage to generate NUP98 and NUP96 fragments, which exhibit distinct functions.
• Subcellularly, NUP98 localizes to the nucleus membrane, nuclear pore complex, and nucleoplasm.
• NUP98 also interacts with HIV-1 capsid and nucleocapsid proteins, potentially facilitating viral integration into the host genome. Related references: PMID:23523133
• Key post-translational modifications include acetylation and isopeptide bond formation, and it is classified as a serine protease.

Experimental Guidance and Technical Tips

• For Western blot (WB), consider optimizing the antibody dilution using a relevant cell line expressing NUP98. An expected band around 198 kDa indicates specific detection.
• Ensure the use of appropriate secondary antibodies and detection systems compatible with rabbit monoclonal antibodies.
• For ELISA applications, validate the antibody under your specific assay conditions, including coating buffers and blocking agents.
• The immunogen corresponds to amino acids 712–863 of human NUP98; thus, the antibody may not detect isoforms lacking this region. Validate in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

At CamelBio, we understand the importance of reliable nuclear pore complex research tools. Our NUP98 Rabbit mAb is part of a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. Whether you need rare target sourcing or technical consultation, CamelBio is your trusted partner from concept to clinic.