Anti-NUP98 Polyclonal Antibody for WB, IF/ICC, ELISA - P52948

Core Product Specifications and Parameters

Biological Background: NUP98 Function and Localization

• NUP98, also known as ADIR2, NUP96, NUP196, or Nup98-96, is a component of the nuclear pore complex (NPC) and is produced as a precursor protein that undergoes autocatalytic cleavage to generate NUP98 and NUP96, both integral to NPC function.
• It plays a critical role in NPC assembly and maintenance, and both NUP98 and NUP96 are required for bidirectional nucleocytoplasmic transport. Related references: PMID:33097660
• In cooperation with DHX9, NUP98 participates in transcription regulation and alternative splicing of specific genes, and is involved in localizing DHX9 to discrete intranuclear foci known as GLFG bodies. Related references: PMID:28221134
• The protein localizes to the nuclear membrane and is predominantly found at the nuclear pore complex, with additional presence in the nucleoplasm.
• Key post-translational modifications include acetylation, phosphorylation, isopeptide bond formation, and ubiquitin-like conjugation, which may regulate its function and interactions.
• NUP98 interacts with HIV-1 capsid protein P24 and nucleocapsid protein P7, potentially facilitating viral integration into the host nucleus, with reduced binding affinity observed for certain capsid mutations. Related references: PMID:23523133
• The protein features FG/GLFG repeat domains that serve as docking sites for transport receptors, enabling selective passage of macromolecules through the NPC.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a region within the NUP98 portion (aa 621-920) of the full-length NUP98 precursor, suggesting the antibody primarily detects the cleaved NUP98 form, but may also recognize the precursor due to sequence overlap.
• For Western blotting, a band around 98 kDa (cleaved NUP98) or 198 kDa (precursor) may be observed depending on sample preparation and electrophoresis conditions; validate using nuclear extracts from human or mouse cells.
• For immunofluorescence/ICC, expect punctate nuclear rim staining characteristic of nuclear pore complexes; permeabilization is necessary for antibody access.
• ELISA applications can be used for quantifying NUP98 levels in cell lysates or purified fractions; optimize coating and detection conditions empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive access to IVD raw materials and technical support, covering every stage from concept to clinic. This anti-NUP98 polyclonal antibody exemplifies our high-quality portfolio for nuclear transport and pore complex research. We also provide validated antibody pairs, bulk reagents, and custom sourcing for rare targets to meet your diagnostic development needs.