Anti-NUP85 Polyclonal Antibody for WB, ELISA - Q9BW27

Core Product Specifications and Parameters

Biological Background: NUP85 Function and Localization

• NUP85 (also known as Nup75, FROUNT, NPHS17) is a 75 kDa component of the nuclear pore complex (NPC), encoded by the NUP85 gene.
• It is an essential scaffolding nucleoporin required for NPC assembly and maintenance, and as part of the Nup107-160 subcomplex, it participates in RNA export and tethering of NUP96/Nup98 and NUP153 to the nucleus.
  Related references: PMID:12718872
• The Nup107-160 complex, including NUP85, is required for spindle assembly during mitosis.
  Related references: PMID:16807356
• NUP85 (also called FROUNT) mediates CCL2-CCR2 signaling by promoting membrane clustering of activated CCR2 and linking to the phosphatidyl-inositol 3-kinase-Rac-lammellipodium protrusion cascade, thereby regulating monocyte chemotaxis.
  Related references: PMID:15995708
• It is involved in kidney development, with variants associated with nephrotic syndrome; plays a role in nephrogenesis.
  Related references: PMID:30179222
• Subcellularly, NUP85 localizes to the nuclear pore complex, but also to the kinetochore, mitotic spindle, and cytoplasm, reflecting its dynamic roles in transport and cell division.
• Post-translational modifications include acetylation and phosphorylation (as per UniProt keywords).
• The gene undergoes alternative splicing, potentially producing multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 427-656) of human NUP85. This region is suitable for generating antibodies that recognize endogenous full-length protein.
• For Western blot (WB), consider using whole cell lysates or nuclear fractions from human, mouse, or rat cells, and validate specificity by signal at ~75 kDa.
• For ELISA, use recombinant NUP85 or peptide as coating antigen.
• Since NUP85 is part of large macromolecular complexes (Nup107-160), Co-IP or other interaction assays may require detergent and buffer optimization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with a comprehensive sourcing platform for IVD raw materials, including validated antibody pairs, monoclonal/polyclonal antibodies, and ancillary reagents. Our portfolio covers diverse targets like nucleoporins for nuclear transport and cell division studies. We also offer expert technical services and consulting to support your development from concept to clinic.