Anti-NUP62 Rabbit Polyclonal Antibody for WB, ELISA - P37198

Core Product Specifications and Parameters

Biological Background: NUP62 Function and Localization

• NUP62, also known as Nuclear pore glycoprotein p62, 62 kDa nucleoporin, or nucleoporin Nup62, is encoded by the NUP62 gene in humans.
• Essential component of the nuclear pore complex, facilitating nucleocytoplasmic transport through its N-terminal domain.
  Related references: PMID:1915414
• The C-terminal coiled-coil region mediates protein-protein interactions, promoting association with centrosomes and may function in anchorage of p62 to the pore complex.
  Related references: PMID:1915414 PMID:24107630
• Plays a critical role in mitotic cell cycle progression by regulating centrosome segregation, centriole maturation and spindle orientation.
  Related references: PMID:24107630
• May be involved in protein recruitment to the centrosome after nuclear breakdown, linking nuclear transport to mitotic regulation.
  Related references: PMID:24107630
• Subcellular localization includes the nuclear pore complex, cytoplasm, spindle pole, centrosome, and nuclear envelope, consistent with its roles in both interphase and mitotic processes.
• Post-translational modifications documented by UniProt include acetylation, phosphorylation, and disulfide bond formation, which may regulate its function.
• Additional keywords suggest involvement in host-virus interaction, mRNA transport, and protein transport; however, these functions may require further validation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 403-522 within the C-terminal region, encompassing coiled-coil domains; consider this when performing epitope mapping or blocking experiments.
• For Western blot, the predicted molecular weight is approximately 53 kDa; ensure that the observed band aligns with this in human-derived samples.
• ELISA performance may depend on proper coating and detection conditions; using the recombinant immunogen as a positive control is advisable.
• Cross-reactivity is reported for human; for studies in other species, perform in silico or empirical validation due to potential sequence divergence.
• For immunofluorescence or other applications not listed, optimization is recommended as the antibody's suitability has not been fully characterized.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated one-stop sourcing bridge for IVD raw materials, CamelBio supports diagnostic developers and researchers with a comprehensive portfolio including validated antibody pairs, optimized polyclonal antibodies like this anti-NUP62 product, and bulk ancillary reagents. Whether you are advancing nuclear transport research or developing assays targeting nucleoporins, our team can facilitate procurement of high-quality raw materials and related technical consulting. Contact us to streamline your supply chain from concept to clinic.