Anti-NUP62 Monoclonal Antibody for WB, IP, ELISA - P37198

Core Product Specifications and Parameters

Biological Background: NUP62 Function and Localization

• NUP62 is an essential component of the nuclear pore complex (NPC), with its N-terminal domain mediating nucleocytoplasmic transport. Related references: PMID:1915414
• The C-terminal region of NUP62 facilitates protein-protein interactions via coiled-coil formation, promoting its anchoring to the NPC and association with centrosomes. Related references: PMID:1915414 PMID:24107630
• It plays a critical role in mitotic progression by regulating centrosome segregation, centriole maturation, and spindle orientation. Related references: PMID:24107630
• After nuclear envelope breakdown, NUP62 may be involved in recruiting proteins to the centrosome. Related references: PMID:24107630
• Subcellularly, NUP62 localizes to the nuclear pore complex, as well as to cytoplasmic structures including centrosomes and spindle poles during mitosis.
• The protein undergoes post-translational modifications such as phosphorylation and acetylation, which may modulate its functions.
• NUP62 is also known as 62 kDa nucleoporin and is subject to glycosylation and disulfide bond formation.

Experimental Guidance and Technical Tips

• For Western blotting, the predicted molecular weight of NUP62 is ~53 kDa; consider using an appropriate gel percentage to resolve the band and confirm with a positive control.
• The immunogen corresponds to the C-terminal region (amino acids 423–522), which is conserved among human, mouse, and rat NUP62, supporting observed cross-reactivity.
• In immunoprecipitation, validate the antibody under your specific lysis conditions, as it may co-immunoprecipitate NUP62-associated complexes.
• For ELISA, the synthetic peptide immunogen can serve as a positive control to optimize assay conditions.
• Enrichment of nuclear or nuclear pore fractions may enhance signal detection due to the primary localization of NUP62.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated one-stop partner for IVD raw-material sourcing, supplying validated antibodies like this anti-NUP62 monoclonal antibody to support research into nucleocytoplasmic transport and mitotic regulation. Beyond individual reagents, we offer matched antibody pairs, optimized bulk ancillary reagents, and customized raw-material procurement to facilitate your diagnostic development from concept to clinic.