Anti-NUP54 Rabbit Polyclonal Antibody for WB, ELISA - Q7Z3B4

Core Product Specifications and Parameters

Biological Background: Nucleoporin p54 Function and Localization

• NUP54 encodes the 54 kDa nucleoporin, a core structural component of the nuclear pore complex (NPC).
• It facilitates bidirectional trafficking of proteins and mRNAs across the nuclear envelope.
• The protein localizes specifically to the nuclear pore complex and the nuclear membrane.
• Alternative splicing generates multiple transcript variants, adding functional diversity.
• NUP54 is glycosylated and contains characteristic FG-repeat regions typical of channel nucleoporins.
• High-resolution structural studies (3D-structure) and proteomics profiling confirm its scaffold role in the NPC.
• Genetic alterations in NUP54 are associated with dystonia, a neurological movement disorder.

Experimental Guidance and Technical Tips

• The immunogen sequence (aa 100–400) covers a conserved central region, likely reactive with full-length and splice variants.
• For western blot, validate the predicted ~55 kDa band in relevant human, mouse, or rat lysates; run appropriate controls.
• For ELISA, consider pairing with a matched capture antibody; optimize coating and detection conditions.
• As a polyclonal antibody, lot-to-lot consistency should be verified; centrifuge before use to avoid aggregates.
• Include blocking and wash steps with standard BSA or milk for WB; follow standard ELISA protocols for plate preparation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research teams with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, high-titer monoclonal/polyclonal antibodies, and bulk ancillary reagents. For nucleoporin targets like NUP54—central to nuclear transport and cell biology—we offer customized sourcing and supply solutions to accelerate your assay development. From rare targets to routine reagents, CamelBio bridges your concept to the clinic with reliable, scalable procurement support.