Anti-NUP50 Rabbit Polyclonal Antibody - Q9UKX7

Core Product Specifications and Parameters

Biological Background: NUP50 Function and Localization

• NUP50 (also known as NPAP60, NPAP60L, or 50 kDa nucleoporin) is a component of the nuclear pore complex (NPC) that directly participates in nuclear protein import.
  Related references: PMID:20016008
• It actively displaces nuclear localization signals (NLSs) from importin-alpha, facilitating the disassembly of the importin-alpha:beta-cargo complex and recycling of importins.
  Related references: PMID:20016008
• NUP50 interacts with cell cycle regulatory proteins such as CDKN1B, and this interaction is required for proper intracellular transport and degradation of CDKN1B.
• The protein localizes to the nuclear membrane and is specifically found at the nuclear pore complex within the nucleus.
• NUP50 is ubiquitously expressed, with highest levels detected in testis, peripheral blood leukocytes, and fetal liver.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitination (isopeptide bond formation), and the gene undergoes alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 340-468 of human NUP50, suggesting the antibody recognizes an internal epitope. Consider validating the reactivity in your target species and tissue type.
• For western blot (WB), the predicted molecular weight is approximately 50 kDa; however, post-translational modifications may cause slight shifts in migration.
• Cross-reactivity with mouse and rat is expected based on supplier data. When using these species, perform a pilot experiment to confirm reactivity and optimal dilution.
• In immunohistochemistry (IHC-P), include both positive and negative tissue controls, as NUP50 is broadly expressed but may vary in abundance.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw-material partner, CamelBio provides diagnostic manufacturers and research laboratories with comprehensive access to validated antibodies, optimized polyclonal and monoclonal reagents, and bulk ancillary materials. For nuclear pore complex targets like NUP50, our supply network can support your development needs—from lead candidate screening to scale-up. Contact us to explore customized sourcing of rare-target antibodies and complementary assay reagents.