Anti-NUP35 Rabbit Polyclonal Antibody for WB and ELISA - Q8NFH5

Core Product Specifications and Parameters

Biological Background: NUP35 Function and Localization

• NUP35 encodes nucleoporin NUP35, also known as 35 kDa nucleoporin, mitotic phosphoprotein 44 (MP-44), nuclear pore complex protein Nup53, and nucleoporin NUP53.
• Functions as a component of the nuclear pore complex (NPC), acting both as a structural component and as a docking or interaction partner for transiently associated nuclear transport factors.
• May play a role in the association of MAD1 (mitotic arrest deficient 1) with the NPC.
• Localizes to the nucleus, specifically the nuclear pore complex and nuclear membrane.
• Post-translationally modified: classified as a phosphoprotein; involved in mRNA transport, protein transport, and translocation.
• Alternative splicing generates multiple isoforms; the three-dimensional structure has been determined.

Experimental Guidance and Technical Tips

• For Western blot, expect a specific band near 35 kDa. Validate with positive control lysates from human, mouse, or rat cells; optimized loading and antibody dilution may be required.
• The immunogen (residues 1‑326) covers the N‑terminal region, so the antibody likely recognizes conserved epitopes across human, mouse, and rat; consider cross‑species validation in your experimental system.
• For ELISA, use recombinant NUP35 protein or cell‑extract coating; pilot tests for coating concentration and blocking conditions are recommended.
• Since NUP35 is a nuclear pore component, immunofluorescence may be feasible in paraformaldehyde‑fixed cells, but this application has not been validated; include appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one‑stop partner for IVD raw materials, offering validated antibody pairs, optimized polyclonal antibodies like this anti‑NUP35, and bulk ancillary reagents to support your nuclear pore complex and nucleocytoplasmic transport research. With our end‑to‑end sourcing capabilities, we connect diagnostic manufacturers and research institutes to the rare‑target raw materials they need, from concept to clinic.