Anti-NUP214 Polyclonal Antibody for WB, IF/ICC, ELISA - P35658

Core Product Specifications and Parameters

Biological Background: NUP214 Function and Localization

• NUP214, also known as CAN protein or 214 kDa nucleoporin, is a large nucleoporin component of the nuclear pore complex (NPC).
• It has a critical role in nucleocytoplasmic transport, mediating the passage of macromolecules between the nucleus and cytoplasm.
  Related references: PMID:9049309 PMID:31178128
• It serves as a docking site for receptor-mediated import of substrates across the NPC.
  Related references: PMID:31178128 PMID:8108440
• During adenovirus infection, NUP214 is required for capsid disassembly and release of the viral genome into the nucleus (in vitro).
• Subcellularly, NUP214 is localized to the nuclear pore complex on the nuclear envelope.
• NUP214 is expressed in a tissue-specific manner, with highest levels in thymus, spleen, bone marrow, kidney, brain, and testis.
• It is subject to various post-translational modifications, including acetylation, phosphorylation, and glycosylation, and is involved in chromosomal rearrangements linked to oncogenesis.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a synthetic peptide within amino acids 1251–1350 of human NUP214; thus, the antibody may recognize an internal epitope conserved across isoforms.
• For western blot, due to the large protein size (~214 kDa), consider using a low-percentage (e.g., 6–8%) SDS-PAGE gel and prolonged transfer times to ensure efficient blotting.
• In immunofluorescence/ICC, validate fixation and permeabilization conditions (e.g., methanol vs. Triton X-100) to achieve optimal nuclear pore staining.
• For ELISA, the peptide-based immunogen suggests this antibody may be suitable for sandwich or indirect ELISA formats; cross-reactivity with mouse and rat should be verified if working with those species.
• Always include appropriate positive and negative controls (e.g., HeLa lysates for WB) and titrate the antibody for each application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw materials, supporting the full development pipeline from concept to clinic. This anti-NUP214 polyclonal antibody complements our portfolio of validated antibodies, antigen/antibody pairs, and ancillary reagents for nuclear pore and intracellular transport research. We also specialize in sourcing rare and custom targets, offering flexible bulk supply to meet the demands of diagnostic manufacturers and reference laboratories.