Anti-NUP210 (GP210/POM210) Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q8TEM1

Core Product Specifications and Parameters

Biological Background: NUP210 Function and Localization

• NUP210, also known as GP210, POM210, or nucleoporin Nup210, is a 205 kDa nuclear pore membrane glycoprotein.
• It plays an essential role in nuclear pore complex assembly, fusion, and spacing, maintaining structural integrity of the nuclear envelope.
• The protein localizes to the nuclear membrane, nuclear pore complex, and endoplasmic reticulum membrane.
• Ubiquitously expressed, with highest levels in lung, liver, pancreas, testis, and ovary, and lower levels in brain, kidney, spleen, heart, and skeletal muscle.
• Post-translational modifications include glycosylation and phosphorylation, and it is predicted to contain a signal peptide and transmembrane helices.
• It is involved in mRNA and protein transport across the nuclear envelope, serving as a critical component of the nucleocytoplasmic transport machinery.

Experimental Guidance and Technical Tips

• For western blot, use a suitable high-molecular-weight range marker and extended transfer conditions due to the large size (~205 kDa) of NUP210.
• In immunofluorescence/immunocytochemistry, optimize fixation (e.g., 4% PFA or methanol) and permeabilization to detect nuclear envelope and pore complex localization.
• The antibody was raised against a recombinant fragment (aa 680-880) of human NUP210; validate performance in your specific experimental system, considering cross-reactivity with mouse.
• For ELISA, use an appropriate coating antigen and verify sensitivity with your target protein.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a one-stop platform for IVD raw materials, supporting projects from concept to clinic. This anti-NUP210 polyclonal antibody addresses nuclear pore biology research, and CamelBio can also supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets. Contact us to streamline your procurement and accelerate assay development.