Anti-NUP205 Rabbit Polyclonal Antibody for WB and ELISA - Q92621

Core Product Specifications and Parameters

Biological Background: NUP205 Function and Localization

• Nuclear pore complex protein Nup205 (205 kDa nucleoporin) is a key structural component of the NPC.
• It plays a critical role in NPC assembly and/or maintenance, as demonstrated by early functional studies (PubMed:9348540).
  Related references: PMID:9348540
• Nup205 anchors NUP62 and other nucleoporins to the NPC, but not NUP153 and TPR, contributing to the architectural integrity of the pore (PubMed:15229283).
  Related references: PMID:15229283
• In association with TMEM209, Nup205 may facilitate the nuclear transport of MYC and other nuclear proteins, implicating it in nucleocytoplasmic trafficking (PubMed:22719065).
  Related references: PMID:22719065
• The protein localizes specifically to the nucleus membrane and the nuclear pore complex, consistent with its functional role.
• Expression of Nup205 is detected in the testis, suggesting tissue-specific regulatory functions.
• According to proteomics data, Nup205 is subject to post-translational modifications including acetylation and phosphorylation.
• Genetic variants in NUP205 have been associated with disease phenotypes, underscoring its importance in normal cellular function.

Experimental Guidance and Technical Tips

• The immunogen for this antibody corresponds to amino acids 10-120 of human NUP205 (N-terminal region). Consider using this antibody to probe the N-terminal portion of the protein in your assays.
• For Western blot applications, consider testing lysates from human, mouse, or rat cell lines or tissues, as cross-reactivity is indicated.
• The reported protein weight of NUP205 is 228 kDa; ensure appropriate gel percentage and transfer conditions for large-molecular-weight proteins.
• ELISA may be used for quantitative detection; validate the assay conditions in your specific experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers, laboratories, and research institutes with high-quality IVD raw materials, technical services, and consulting support from concept to clinic. This anti-NUP205 rabbit polyclonal antibody supports research on nuclear pore complex biology and nucleocytoplasmic transport, areas critical for understanding cellular trafficking mechanisms. Our comprehensive supply portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and specialized sourcing for rare targets—all designed to streamline your development process.