Anti-NUP155 Rabbit Polyclonal Antibody for WB, ELISA - O75694

Core Product Specifications and Parameters

Biological Background: NUP155 Function and Localization

• NUP155 (Nuclear pore complex protein Nup155, also termed 155 kDa nucleoporin) is an essential scaffold component of the nuclear pore complex, facilitating bidirectional nucleocytoplasmic transport of proteins and mRNAs.
• Encoded by the NUP155 gene, multiple isoforms are produced through alternative splicing.
• It is localized to the nuclear pore complex embedded in the nuclear envelope, spanning both the inner and outer nuclear membranes.
• Post-translational modifications include glycosylation, phosphorylation, disulfide bond formation, isopeptide bond formation, and ubiquitin-like conjugation.
• NUP155 is ubiquitously expressed in human tissues, with detected expression in heart, brain, placenta, lung, liver, skeletal muscle, kidney, and pancreas.
• Genetic variations in NUP155 are associated with atrial fibrillation (ATFB15), highlighting its clinical relevance in cardiac electrophysiology.

Experimental Guidance and Technical Tips

• The expected molecular weight of NUP155 is approximately 155 kDa. For Western blotting, use a low-percentage acrylamide gel (e.g., 6-8%) and optimized transfer conditions for high molecular weight proteins.
• This rabbit polyclonal antibody was raised against a recombinant fragment (aa 1102-1391); it may recognize multiple isoforms. Validate specificity using appropriate controls in your model system.
• For ELISA applications, consider optimizing coating buffers and blocking agents to minimize background, given the polyclonal nature.
• Cross-reactivity is reported for human samples; performance in other species should be experimentally validated.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted one-stop partner for IVD raw material procurement, supporting diagnostic manufacturers and research laboratories worldwide. For NUP155, a key component of the nuclear pore complex with roles in transport and disease, we offer validated polyclonal antibodies like this one, along with matched antigens, bulk ancillary reagents, and customized sourcing of rare-target raw materials. Whether you need antibody pairs, optimized monoclonals, or comprehensive assay development support, CamelBio bridges the gap from concept to clinic.