Anti-NUP153 Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P49790

Core Product Specifications and Parameters

Biological Background: Nuclear pore complex protein Nup153 Function and Localization

• NUP153 encodes a 153 kDa nucleoporin (also known as Nup153) that is a critical scaffolding component of the nuclear pore complex (NPC), essential for normal nucleocytoplasmic transport of proteins and mRNAs.
• Functions in the nuclear phase of the NPC, contributing to the quality control and retention of unspliced mRNAs; together with TPR, it regulates nuclear export of unspliced mRNA species containing constitutive transport elements (CTE) in an NXF1- and KHDRBS1-independent manner.
• Mediates the anchoring of TPR to the nuclear membrane at the NPC, with its repeat-containing domain possibly anchoring other NPC components to the pore membrane.
• Possesses a zinc-finger domain and exhibits DNA-binding activity, suggesting a role as a DNA-binding subunit of the NPC.
• Subcellular localization is mainly at the nucleus, nuclear membrane, and specifically the nuclear pore complex.
• Involved in host-virus interactions: interacts with HIV-1 capsid protein p24 to facilitate viral integration into the nucleus of non-dividing cells, and binds HIV-2 protein vpx to promote nuclear translocation of the lentiviral genome.
• Post-translationally modified by acetylation, phosphorylation, isopeptide bond formation, and ubiquitin-like conjugation, which may regulate its function and localization.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-100) of human NUP153; this region may be conserved across species, supporting observed cross-reactivity with mouse and rat. Verify sequence homology before use in other species.
• For Western blotting, the predicted molecular weight is approximately 154 kDa; ensure appropriate gel percentage and transfer conditions for high molecular weight proteins.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), nuclear pore complex staining is expected; include appropriate nuclear counterstains and controls.
• ELISA may be used for antibody titration or screening; consider pairing with other NUP153-targeting reagents for sandwich assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with one-stop access to validated IVD raw materials, including monoclonal antibodies like this anti-NUP153 clone, optimized for applications such as WB, IHC, IF, and ELISA. We also offer custom antibody development, bulk ancillary reagents, and rare target sourcing to support nuclear pore complex and host-pathogen interaction research. Let CamelBio streamline your transition from concept to clinic with reliable, high-quality reagents.