Anti-NUMB Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P49757

Core Product Specifications and Parameters

Biological Background: NUMB Function and Localization

• NUMB functions as an endocytic adaptor protein that regulates clathrin-mediated receptor endocytosis, influencing cell fate decisions. Related references: PMID:18657069
• It plays a critical role in neurogenesis by maintaining neural progenitor cells (radial glial cells) during embryonic development, allowing daughter cells to choose progenitor over neuronal fate.
• NUMB is required throughout embryonic neurogenesis but not for the initial proliferation of neural progenitor cells before the onset of neurogenesis.
• Postnatally, NUMB contributes to subventricular zone (SVZ) neurogenesis by regulating neuroblast survival and ependymal wall integrity, and may mediate local repair of brain ventricular wall damage.
• The protein localizes to the cell membrane and endosome membrane, consistent with its role in endocytic trafficking.
• NUMB undergoes alternative splicing and post-translational modifications, including phosphorylation and ubiquitin conjugation, which modulate its activity.
• It is a conserved developmental protein across human, mouse, and rat, with established roles in cell fate specification.

Experimental Guidance and Technical Tips

• The antibody was raised against a recombinant fragment (aa 521-640) of human NUMB. For western blot, anticipate a specific band around 71 kDa; validate using appropriate positive and negative controls.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), perform antigen retrieval optimization and titrate the antibody dilution, as staining may vary with fixation and tissue type.
• As a polyclonal antibody, lot-to-lot consistency should be confirmed; consider running a standard curve with each new lot.
• Cross-reactivity with mouse and rat NUMB has been reported, but sequence alignment suggests potential reactivity with other species; verify in your experimental system if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, providing validated antibody pairs, optimized polyclonal antibodies like this anti-NUMB antibody, and bulk ancillary reagents. Our team specializes in neurobiology and cell signaling targets, offering seamless support from target selection to custom antibody development. Contact us to streamline your diagnostic assay workflow with high-quality NUMB reagents and expert technical consultation.