Anti-NUMB Rabbit Monoclonal Antibody for WB, IP, ELISA - P49757

Core Product Specifications and Parameters

Biological Background: NUMB Function and Localization

• Protein numb homolog (NUMB), also known as S171, is encoded by the NUMB gene (GeneID: 8650) and is evolutionarily conserved.
• NUMB regulates clathrin-mediated receptor endocytosis, crucial for internalizing cell-surface receptors. Related references: PMID:18657069
• Essential for embryonic neurogenesis: maintains radial glial cells (RGCs) by enabling daughter cells to adopt progenitor fates over neuronal differentiation (By similarity).
• Postnatally, NUMB functions in subventricular zone (SVZ) neurogenesis, promoting neuroblast survival and maintaining ependymal wall integrity (By similarity).
• May also participate in repairing brain ventricular wall damage (By similarity).
• Subcellularly localized to the cell membrane and endosome membrane, consistent with its role in endocytosis.
• Subject to post-translational modifications: annotated as a phosphoprotein and modified by ubiquitin-like conjugation (Ubl conjugation).
• Keywords from UniProt: 3D-structure, Alternative splicing, Developmental protein, Phosphoprotein.

Experimental Guidance and Technical Tips

• The immunogen design targeting the N-terminal 1–100 amino acids may yield antibodies recognizing multiple isoforms if alternative splicing occurs within this region; validate specificity in your assay system.
• For Western blotting, consider the predicted molecular weight of 71 kDa (UniProt: 70.8 kDa) and optimize sample preparation to detect potential post-translational modifications or splice variants.
• When using for immunoprecipitation (IP), verify compatibility with your lysis buffer and ensure minimal interference with the target's native conformation.
• In ELISA applications, the peptide immunogen can serve as a positive control to confirm antibody binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a one-stop supply channel for IVD raw materials, empowering diagnostic developers, laboratories, and research institutes with seamless access to validated reagents. For neurobiology and cell signaling research targeting NUMB, we provide optimized antibody pairs, monoclonal antibodies, and bulk ancillary reagents, alongside rare target sourcing solutions to accelerate your projects from concept to clinic.