Anti-NUMA1 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q14980

Core Product Specifications and Parameters

Biological Background: NUMA1 Function and Localization

• Nuclear Mitotic Apparatus Protein 1 (NUMA1), also known as NuMA, NMP-22, or SP-H antigen, is a large 238 kDa coiled-coil protein encoded by the NUMA1 gene. It is a major component of the nuclear matrix.
• NUMA1 plays a critical role in the formation and maintenance of spindle poles during mitosis by tethering microtubule minus ends, thereby ensuring proper chromosome alignment and segregation.
  Related references: PMID:11956313 PMID:12445386
• It functions in mitotic spindle orientation and elongation through dynein-dynactin-dependent mechanisms, interacting with cortical complexes containing GPSM2 and Gα proteins, as well as phosphoinositides like PI(4,5)P2.
  Related references: PMID:22327364 PMID:23027904 PMID:23921553 PMID:24371089 PMID:24996901 PMID:23870127 PMID:24109598
• NUMA1 is also required for mitotic microtubule aster assembly and anastral spindle formation, positively regulating TNKS localization to spindle poles.
  Related references: PMID:11163243 PMID:11229403 PMID:12445386 PMID:25657325 PMID:16076287
• Beyond mitosis, NUMA1 is a highly abundant nuclear matrix protein that occupies the majority of the nuclear volume, suggesting a structural role. It is essential for epidermal differentiation and hair follicle morphogenesis.
• Subcellular localization includes the nucleus, nucleoplasm, nuclear matrix, spindle poles, centrosomes, cell cortex, and lateral cell membrane, reflecting its dynamic roles in both interphase and mitosis.
• Post-translational modifications include acetylation, ADP-ribosylation, phosphorylation, and ubiquitination, as annotated in UniProt (isopeptide bond, Ubl conjugation, glycoprotein, lipoprotein), which may regulate its functions.

Experimental Guidance and Technical Tips

• Given the large molecular weight of NUMA1 (approximately 238 kDa), for Western blotting, consider using a low-percentage polyacrylamide gel (e.g., 6–8%) and extended transfer times to ensure efficient transfer.
• The immunogen corresponds to the N-terminal region (amino acids 1–309) of human NUMA1; verify that this region is not occluded by post-translational modifications or binding partners in your samples.
• For immunofluorescence (IF/ICC), NUMA1 displays nuclear localization in interphase cells and spindle pole localization in mitotic cells; optimize fixation and permeabilization conditions to preserve antigenicity.
• In immunohistochemistry (IHC), antigen retrieval methods may be necessary for formalin-fixed paraffin-embedded tissues; validate staining patterns against known subcellular localizations.
• As a polyclonal antibody, lot-to-lot variability can occur; include appropriate positive and negative controls in each experiment.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers, research laboratories, and core facilities with a streamlined one-stop solution for IVD raw materials—from validated antibody pairs and optimized polyclonal antibodies to bulk ancillary reagents and rare-target sourcing. For NUMA1 research, this product supports investigations into mitotic spindle dynamics, chromosome segregation, and nuclear matrix organization. Partner with CamelBio to bridge your procurement needs from concept to clinic.