Anti-NUMA1 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - Q14980

Core Product Specifications and Parameters

Biological Background: NUMA1 Function and Localization

• NUMA1 (Nuclear Mitotic Apparatus Protein 1), also known as NMP-22 or NuMA, is a large coiled-coil protein essential for mitotic spindle organization and chromosome segregation.
• It tethers microtubule minus ends at spindle poles, which is critical for establishing and maintaining spindle pole integrity. Related references: PMID:11956313 PMID:12445386
• NUMA1 regulates spindle orientation and elongation during metaphase and anaphase via a dynein-dynactin-dependent mechanism, involving interactions with GPSM2 and G(i) alpha proteins at the cell cortex. Related references: PMID:22327364 PMID:23027904 PMID:23921553 PMID:24109598 PMID:23870127 PMID:24996901
• During anaphase, NUMA1 recruits dynein-dynactin to polar cortical regions through direct binding to phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2), facilitating spindle elongation and chromosome segregation. Related references: PMID:24371089 PMID:22327364 PMID:23921553 PMID:24996901
• Beyond mitosis, NUMA1 is a major structural constituent of the nuclear matrix, occupying a large volume of the interphase nucleus and possibly serving a non-mitotic scaffolding role. Related references: PMID:10075938
• It is required for epidermal differentiation and hair follicle morphogenesis, indicating roles in tissue development.
• Subcellular localization dynamically shifts between nucleoplasm, spindle poles, centrosomes, cell cortex, and lateral cell membrane during the cell cycle.
• Post-translational modifications include acetylation, ADP-ribosylation, phosphorylation, and ubiquitination; it is also a lipid-binding lipoprotein and glycoprotein.

Experimental Guidance and Technical Tips

• The immunogen lies within the C-terminal region (aa 2000–2100) of human NUMA1; consider that the antibody may detect both full-length protein and possible C-terminal fragments.
• For Western blotting (WB), note the high molecular weight (~238 kDa); use low-percentage gels, extended transfer times, and confirm that protein ladders are properly resolved.
• In immunohistochemistry (IHC-P), expect nuclear staining in interphase cells and spindle-pole accumulation in mitotic cells; validate with appropriate cell-cycle markers or synchronized cultures.
• Cross-reactivity is indicated for human, mouse, and rat; verify reactivity in your target species and sample type before critical experiments.
• For ELISA applications, optimize coating concentrations and include relevant controls to ensure specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive range of IVD raw materials, customized antibody development, and technical support to streamline your diagnostic workflow. This anti-NUMA1 monoclonal antibody supports research in mitotic cell division and nuclear architecture, serving as a reliable tool for assay development. From validated antibody pairs and optimized monoclonals to bulk ancillary reagents, CamelBio helps bridge the gap between concept and clinic.