Anti-NUF2 Rabbit Monoclonal Antibody for WB, ELISA - Q9BZD4

Core Product Specifications and Parameters

Biological Background: NUF2 Function and Localization

• NUF2 is a core component of the NDC80 complex, crucial for proper chromosome segregation and spindle checkpoint signaling. Related references: PMID:12438418 PMID:14654001 PMID:15062103
• NUF2 is required for maintaining kinetochore integrity and establishing stable microtubule binding sites at the outer kinetochore. Related references: PMID:15548592
• Together with the SKA1 complex, the NDC80 complex enhances microtubule binding and aids in tracking depolymerizing microtubules. Related references: PMID:23085020
• Subcellular localization: predominantly found at the centromere and kinetochore regions of chromosomes during mitosis.
• Post-translational modifications: NUF2 is subject to phosphorylation and acetylation, which may regulate its function during cell division.
• Alternatively known as CDCA1 (Cell division cycle-associated protein 1), NUF2 is a coiled-coil protein essential for proper chromosome alignment.

Experimental Guidance and Technical Tips

• For Western blotting, the expected band size is ~54 kDa; we recommend using whole cell lysates from human cell lines expressing NUF2.
• For ELISA, the synthetic peptide immunogen can serve as a positive control to assess antibody reactivity.
• As a rabbit monoclonal antibody, it provides consistent batch-to-batch reproducibility and high affinity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop sourcing partner for IVD manufacturers, laboratories, and research institutes, offering comprehensive access to raw materials, technical services, and consulting from concept to clinic. We supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and specialized raw-material sourcing for rare targets like NUF2, supporting your kinetochore and cell division research. With our B2B-focused approach, you can streamline procurement and accelerate your diagnostic assay development.