Anti-NUDT5 Monoclonal Antibody for WB, IF/ICC, ELISA - Q9UKK9

Core Product Specifications and Parameters

Biological Background: NUDT5 Function and Localization

• NUDT5 is a dual-function enzyme: in the absence of diphosphate, it acts as an ADP-sugar pyrophosphatase, hydrolyzing various modified nucleoside diphosphates; in the presence of diphosphate, it catalyzes ATP synthesis. Related references: PMID:27257257
• As an ADP-sugar pyrophosphatase, NUDT5 hydrolyzes substrates such as ADP-ribose, ADP-mannose, ADP-glucose, 8-oxo-GDP, and 8-oxo-dGDP with similar efficiencies. Related references: PMID:10567213, PMID:10722730, PMID:17052728
• In the nucleus, NUDT5 mediates ATP synthesis by converting ADP-ribose to ATP and ribose 5-phosphate, a reaction dependent on its dephosphorylation at Thr-45. Related references: PMID:27257257
• Nuclear ATP generation by NUDT5 is critical for energy-consuming chromatin remodeling events. Related references: PMID:27257257
• NUDT5 exhibits in vitro decapping activity on UDP-GlcNAc-capped RNA, cleaving the pyrophosphate bridge of the sugar cap. Related references: PMID:38716860
• NUDT5 binds U8 snoRNA but does not decap it, suggesting a role in RNA metabolism beyond its enzymatic activities.
• The protein localizes to the nucleus and is widely expressed, with highest levels in liver.

Experimental Guidance and Technical Tips

• Since the immunogen corresponds to the N-terminal region (aa 1–100), the antibody is expected to recognize full-length NUDT5 and potentially any isoforms containing this region; always validate in your cell or tissue system.
• For Western blot, a starting dilution of 1:1000 is commonly used for monoclonal antibodies, but optimization is recommended using a dilution series.
• In immunofluorescence/ICC, permeabilization with Triton X-100 or similar detergent is required to access nuclear NUDT5; methanol fixation may also help preserve antigenicity.
• Pre-adsorption with the immunizing peptide can serve as a specificity control in both WB and IF/ICC.
• Cross-reactivity with mouse and rat has been reported; however, sequence alignment of the N-terminal region may help predict reactivity with other species.
• For ELISA, a direct coating of antigen and antibody titration is suggested; consider using recombinant NUDT5 protein for standard curves.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop IVD raw-material sourcing partner that supports diagnostic manufacturers and research laboratories with validated antibodies, bulk reagents, and customization services. For researchers studying nuclear metabolism, epigenetics, or DNA damage repair, CamelBio offers a reliable supply of NUDT5 antibodies and complementary tools. Whether you need optimized monoclonal/polyclonal antibodies, antibody pairs, or rare target sourcing, CamelBio bridges the gap from concept to clinical application.