Anti-NUDT21 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - O43809

Core Product Specifications and Parameters

Biological Background: NUDT21 Function and Localization

• NUDT21 (also known as CPSF5, CFIm25, or Nudix hydrolase 21) is the 25 kDa subunit of the cleavage factor Im (CFIm) complex, which activates pre-mRNA 3' end cleavage and polyadenylation, essential for mRNA maturation. Related references: PMID:14690600, PMID:15937220, PMID:17024186
• It specifically binds to the 5'-UGUA-3' sequence elements in pre-mRNAs, recruiting cleavage and polyadenylation complexes to polyadenylation signals. Related references: PMID:14690600, PMID:17024186, PMID:8626397, PMID:15169763
• NUDT21 acts as a key regulator of alternative cleavage and polyadenylation (APA), influencing 3' UTR length and gene expression diversity. Related references: PMID:17098938, PMID:23187700, PMID:29276085, PMID:20695905
• It forms a homodimer that simultaneously recognizes two 5'-UGUA-3' elements, enhancing binding specificity and processing efficiency. Related references: PMID:20479262, PMID:21295486
• NUDT21 binds adenosine nucleotides without hydrolyzing them, suggesting a non-catalytic role in RNA processing. Related references: PMID:18445629
• Subcellularly, NUDT21 is predominantly nuclear but also detected in the cytoplasm, consistent with its roles in mRNA processing and transport.
• It is broadly expressed across tissues, including heart, brain, placenta, lung, liver, skeletal muscle, kidney, and pancreas.
• Post-translational modifications include acetylation, methylation, and phosphorylation, which may regulate its function in RNA processing and cell differentiation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to full-length human NUDT21 (amino acids 1–227), which may provide robust detection in applications such as Western blot. Consider validating in samples with known NUDT21 expression (e.g., HeLa cells or tissues like liver or kidney).
• For Western blot, a band around 26 kDa is expected. Optimize blocking and antibody dilution for your specific sample type.
• In immunohistochemistry (IHC-P), antigen retrieval conditions should be empirically determined. Include positive control tissues that express NUDT21, such as heart or placenta.
• For ELISA, this polyclonal antibody may be used as a capture or detection reagent. Validate the assay with recombinant NUDT21 protein or biological samples.
• Note that as a polyclonal antibody, batch-to-batch variability may occur; include consistent lot-matched controls in your experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted one-stop partner for IVD raw material procurement, offering validated antibodies, bulk ancillary reagents, and customized sourcing for research and diagnostic development. The NUDT21 polyclonal antibody supports studies in pre-mRNA processing, alternative polyadenylation, and gene regulation. Whether you need optimized antibody pairs, large-scale production, or access to hard-to-find targets, CamelBio’s integrated supply chain ensures reliable access to raw materials from discovery through clinical phases.