Anti-NUDT2 Polyclonal Antibody for WB, ELISA - P50583

Core Product Specifications and Parameters

Biological Background: NUDT2 Function and Localization

• NUDT2 encodes Bis(5'-nucleosyl)-tetraphosphatase asymmetrical, also known as Ap4A hydrolase or Nudix motif 2, a member of the Nudix hydrolase superfamily.
• It catalyzes the asymmetric hydrolysis of diadenosine 5',5'''-P1,P4-tetraphosphate (Ap4A) to produce AMP and ATP, regulating intracellular dinucleoside polyphosphate levels.
• In vitro assays have demonstrated decapping activity toward FAD-capped RNAs and dpCoA-capped RNAs, suggesting a role in RNA metabolism and surveillance.
• The enzyme contains a conserved Nudix motif responsible for nucleotide substrate binding and hydrolysis, with GTP-binding capability noted.
• Post-translational modifications include acetylation, which may influence enzyme activity or stability.
• Mutations in NUDT2 have been associated with neurological disorders such as intellectual disability and neuropathy, highlighting its physiological importance.
• The protein has a predicted molecular weight of approximately 17 kDa (16829 Da) and is conserved across human, mouse, and rat species.

Experimental Guidance and Technical Tips

• For Western blotting, the antibody detects a band at ~17 kDa corresponding to endogenous NUDT2; appropriate positive control lysates (e.g., HeLa, HEK293, or rodent tissue) are recommended.
• Since it is a polyclonal antibody, lot-to-lot consistency should be verified for critical experiments; consider using reduction/denaturation conditions as standard.
• In ELISA applications, the antibody may be used as a capture or detection reagent; optimal working dilutions must be determined empirically and validated against known standards.
• Cross-reactivity with mouse and rat NUDT2 allows for translational studies, but species-specific sequence variations should be confirmed.
• Validate specificity by siRNA knockdown or CRISPR knockout models where feasible, and include negative controls to rule out non-specific binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and research laboratories to provide a comprehensive range of IVD raw materials. For NUDT2-related studies focusing on nucleotide metabolism, RNA biology, or neurological disease biomarkers, we supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and customized sourcing for rare target raw materials. Our integrated supply chain and technical support simplify your development workflow from concept to clinical application.