Anti-NUDT19 Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - A8MXV4

Core Product Specifications and Parameters

Biological Background: NUDT19 Function and Localization

• NUDT19 (Acyl-coenzyme A diphosphatase) is a member of the Nudix hydrolase superfamily, encoded by the NUDT19 gene, and also known as RP2.
• It catalyzes the hydrolysis of fatty acyl-CoA esters to produce acyl-4'-phosphopantetheine and adenosine 3',5'-bisphosphate, with a preference for medium-chain and unsaturated substrates.
• In addition to its acyl-CoA diphosphatase activity, NUDT19 exhibits decapping activity toward dpCoA-capped RNAs in vitro, suggesting a role in RNA metabolism.
• The enzyme localizes to the peroxisome, an organelle central to fatty acid β-oxidation and lipid metabolism.
• NUDT19 requires divalent metal ions such as Mg²⁺ or Mn²⁺ for its catalytic activity, consistent with other Nudix hydrolases.
• It is part of the human reference proteome and has been identified in proteomics studies.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 60-190, a region likely containing conserved catalytic motifs; consider mapping the epitope if needed.
• For Western blot, expect a band near 42 kDa; use peroxisome-rich tissues (e.g., liver) as positive control and validate with knockdown or overexpression.
• In IHC-P and IF/ICC, optimize permeabilization for peroxisomal targets; co-stain with a peroxisomal marker (e.g., PMP70) to confirm localization.
• For ELISA applications, pair this antibody with a suitable detection antibody; titrate both to determine optimal working concentrations.
• Validate cross-reactivity in rat samples using appropriate tissue lysates or cell lines; species-specific protein differences may affect binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw-material sourcing partner, connecting diagnostic manufacturers and research laboratories with validated reagents like this NUDT19 rabbit monoclonal antibody. Our offering supports studies in peroxisomal disorders and lipid metabolism, with access to optimized antibodies, bulk ancillary reagents, and rare target sourcing. From concept to clinic, CamelBio streamlines your procurement with reliable, one-stop solutions.