Anti-NUDT18 Polyclonal Antibody for WB, ELISA - Q6ZVK8

Core Product Specifications and Parameters

Biological Background: NUDT18 Function and Localization

• NUDT18 (MutT homolog 3) is a member of the Nudix hydrolase family, functioning as an 8-oxo-dGDP phosphatase that specifically hydrolyzes oxidized guanine nucleoside diphosphates.
• It preferentially acts on 8-oxo-7,8-dihydroguanine-containing diphosphates (8-oxo-dGDP and 8-oxo-GDP) over their triphosphate counterparts, with negligible activity on unmodified nucleotides, thus sanitizing DNA and RNA precursor pools.
• The enzyme also hydrolyzes 2-hydroxy-dADP and 8-OH-dADP, broadening its substrate range to oxidized adenine derivatives.
• In vitro activity extends to isoprene pyrophosphates, including geranyl diphosphate and farnesyl diphosphate, hinting at a role in isoprenoid metabolism. Related references: PMID:38944687
• Alternative splicing produces multiple transcript variants, which may influence enzymatic properties or expression patterns.
• Catalysis depends on divalent metal ions (Mg²⁺ or Mn²⁺), consistent with other Nudix hydrolases.
• Subcellular localization remains to be fully determined; sequence analysis suggests a cytoplasmic and/or nuclear distribution relevant to nucleotide pool maintenance.

Experimental Guidance and Technical Tips

• For Western blotting, a band of approximately 36 kDa is expected based on the immunogen and predicted molecular weight; verify the exact size in your sample system as post-translational modifications or isoforms may affect migration.
• Validate antibody specificity using positive controls (e.g., human cell lines known to express NUDT18) and consider including a negative control (knockdown or knockout) to confirm band identity.
• In ELISA applications, titrate the antibody to determine the optimal working concentration for your antigen coating and detection setup.
• Due to its polyclonal nature, the antibody may recognize multiple epitopes; perform initial testing to assess background and cross-reactivity with closely related Nudix family members.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutions with streamlined access to high-quality IVD raw materials, including validated antibodies, recombinant proteins, and ancillary reagents. Our integrated supply network supports every stage from concept to clinic, covering bulk procurement, technical consultation, and rare-target sourcing. Whether you need optimized polyclonal antibodies for DNA damage studies or complementary reagents for nucleotide metabolism assays, CamelBio is your dedicated partner in advancing diagnostic and research innovations.