Anti-NUDT16 Polyclonal Antibody for WB, ELISA - Q96DE0

Core Product Specifications and Parameters

Biological Background: NUDT16 Function and Localization

• NUDT16 is a multifunctional enzyme, annotated as U8 snoRNA-decapping enzyme, and also known as IDP phosphatase (IDPase), nudix hydrolase 16, and U8 snoRNA-binding protein H29K.
• In its role as an RNA decapping enzyme, NUDT16 catalyzes the metal-dependent cleavage of the cap structure of U8 snoRNA and mRNAs, essential for rRNA maturation and mRNA turnover. Related references: PMID:20385596, PMID:26121039
• The enzyme also functions as a nucleotide sanitizer, hydrolyzing non-canonical purine nucleotides (IDP, dITP, GDP, dGDP, XDP, ITP, dITP) to their monophosphate forms, with substrate preference IDP > dIDP >> GDP = dGDP > XDP = ITP = dITP, thus preventing their incorporation into nucleic acids. Related references: PMID:20385596
• NUDT16 localizes to multiple compartments including the nucleus, nucleoplasm, nucleolus, and cytoplasm, reflecting its diverse functions in RNA processing and nucleotide metabolism.
• The protein is widely expressed with highest levels in lung, kidney, adrenal gland, testis, heart, and brain.
• NUDT16 requires divalent metal ions such as manganese or cobalt for catalytic activity and binds GTP, ITP, and XTP strongly.
• Beyond canonical cap structures, NUDT16 exhibits decapping activity toward NAD-capped and FAD-capped RNAs, and reportedly toward dpCoA-capped RNAs. Related references: PMID:32432673

Experimental Guidance and Technical Tips

• The immunogen targets amino acids 60–130 within the Nudix domain of human NUDT16. This region shows high conservation between human and mouse, consistent with the cross-reactivity data.
• For Western blot detection, use moderate amounts of whole cell lysate from human or mouse cell lines (e.g., 20–30 µg), and probe with standard chemiluminescent protocols. A band near 21 kDa is expected.
• In ELISA applications, the antibody may serve as a capture or detection reagent. Optimization of coating concentration and paired detection system is recommended.
• Because NUDT16 is a low-abundance protein in some cell types, enrichment of nuclear fractions or use of more sensitive detection reagents may improve signal.
• For functional studies, note that NUDT16 binds RNA and metal ions; the antibody likely recognizes total NUDT16 irrespective of its binding state.

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