Anti-NUDT15 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q9NV35

Core Product Specifications and Parameters

Biological Background: NUDT15 Function and Localization

• NUDT15, also known as MTH2 or nudix hydrolase 15, belongs to the Nudix (nucleoside diphosphate-linked moiety X) hydrolase superfamily and functions as a nucleotide triphosphate diphosphatase.
• Catalyzes the hydrolysis of nucleoside triphosphates including dGTP, dTTP, dCTP, their oxidized forms (e.g., 8-oxo-dGTP), and thiopurine prodrug derivatives 6-thio-dGTP and 6-thio-GTP. Related references: PMID:26238318
• May also hydrolyze some nucleoside diphosphate derivatives. Related references: PMID:22556419 PMID:26238318
• Hydrolyzes oxidized nucleoside triphosphates such as 8-oxo-dGTP in vitro, though the physiological relevance as a sanitizing enzyme remains unclear. Related references: PMID:22556419 PMID:26238318
• Through hydrolysis of thioguanosine triphosphates, may participate in the catabolism of thiopurine drugs. Related references: PMID:25108385 PMID:26238318
• May have a role in DNA synthesis and cell cycle progression by stabilizing PCNA. Related references: PMID:19419956
• Exhibits decapping activity towards dpCoA-capped RNAs in vitro.
• Catalyzes the hydrolysis of isoprene pyrophosphates such as geranyl diphosphate and farnesyl diphosphate in vitro, potentially contributing to the regulation of isoprenoid synthesis and protein isoprenylation. Related references: PMID:38944687

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1-164 of human NUDT15, covering the full-length protein. This antibody is expected to recognize the native, full-length target in appropriate sample types.
• For Western blotting, a band near 19 kDa is expected. Consider routine validation steps, such as positive and negative controls, to confirm specificity in your lysate of interest.
• For immunohistochemistry, antigen retrieval optimization (e.g., heat-induced epitope retrieval in citrate or EDTA buffer) may improve staining results. Titrate antibody concentration to achieve optimal signal-to-background ratio.
• This polyclonal antibody cross-reacts with mouse and rat NUDT15, making it suitable for studies in rodent models.
• As with any polyclonal antibody, lot-to-lot consistency should be verified for critical quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This NUDT15 polyclonal antibody supports research in nucleotide metabolism, thiopurine drug response, and DNA damage repair pathways. As a comprehensive supplier, CamelBio offers validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to streamline your IVD development.