Anti-NUDT15 Polyclonal Antibody for WB, IHC-P, ELISA - Q9NV35

Core Product Specifications and Parameters

Biological Background: NUDT15 Function and Localization

• NUDT15 (nucleotide triphosphate diphosphatase), also known as MutT homolog 2 (MTH2) or Nudix hydrolase 15, catalyzes the hydrolysis of nucleoside triphosphates such as dGTP, dTTP, dCTP, their oxidized forms (e.g., 8-oxo-dGTP), and thiopurine prodrug derivatives like 6-thio-dGTP and 6-thio-GTP. It can also hydrolyze some nucleoside diphosphate derivatives; however, its low efficiency toward oxidized nucleotides in vitro makes its physiological sanitizing role unclear. Related references: PMID:26238318 PMID:22556419

• NUDT15 participates in the catabolism of thiopurine drugs (e.g., azathioprine, 6-mercaptopurine) by hydrolyzing their active thioguanosine triphosphate metabolites, thereby influencing drug efficacy and toxicity. Related references: PMID:25108385 PMID:26238318

• The enzyme may play a role in DNA synthesis and cell cycle progression by stabilizing proliferating cell nuclear antigen (PCNA). Related references: PMID:19419956

• NUDT15 exhibits decapping activity toward dpCoA-capped RNAs in vitro, suggesting involvement in RNA metabolism.

• It can hydrolyze isoprene pyrophosphates such as (2E)-geranyl diphosphate, isopentenyl diphosphate, (2E,6E)-farnesyl diphosphate, and dimethylallyl diphosphate, potentially regulating cellular levels of metabolites essential for isoprenoid synthesis and protein isoprenylation. Related references: PMID:38944687

• NUDT15 is a metal-dependent hydrolase that requires magnesium or manganese for catalytic activity, as highlighted by structural studies and its classification in the Nudix superfamily.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full-length human NUDT15 protein (aa 1–164), so the antibody is expected to recognize the native conformation and linear epitopes. Consider using it for detecting endogenous NUDT15 in whole cell or tissue lysates.
• For Western blotting, a band near 19 kDa is anticipated. Validate specificity using a NUDT15 knockout or knockdown control if available.
• In immunohistochemistry (IHC-P), antigen retrieval conditions may require optimization; frozen sections could provide stronger signal if paraffin-embedded tissues yield low reactivity.
• The antibody cross-reacts with mouse and rat, making it suitable for rodent model studies. Always include appropriate species-specific secondary antibody controls.
• For ELISA, the polyclonal nature may enhance capture sensitivity, but pairing with a monoclonal detector is advised to reduce background.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, from validated antibody pairs and optimized polyclonal antibodies to bulk ancillary reagents. For NUDT15-related research—spanning pharmacogenomics, thiopurine toxicity, and cell cycle regulation—CamelBio streamlines procurement of rare-target raw materials and offers technical support to advance your assay development from concept to clinic.