Anti-NUDC Rabbit Monoclonal Antibody for WB and ELISA - Q9Y266

Core Product Specifications and Parameters

Biological Background: NUDC Function and Localization

• Nuclear migration protein nudC (NUDC), also known as Nuclear distribution protein C homolog, is encoded by the NUDC gene and belongs to a family involved in nuclear movement and cell division.
• Plays a role in neurogenesis and neuronal migration, as inferred from homology studies (By similarity).
• Essential for correct mitotic spindle formation and chromosome segregation during mitosis, ensuring genomic stability. Related references: PMID:12679384 PMID:12852857 PMID:25789526
• Required for cytokinesis and cell proliferation, linking microtubule dynamics to successful cell division. Related references: PMID:12679384 PMID:12852857
• Localizes to multiple subcellular structures including the cytoplasm, cytoskeleton, nucleus, mitotic spindle, and midbody, reflecting its dynamic role in cell cycle progression.
• Ubiquitously expressed across tissues, with high levels in fetal and adult brain, liver, kidney, lung, pancreas, prostate, skeletal muscle, heart, and placenta.
• Post-translational modifications include acetylation and phosphorylation, and the protein features a coiled-coil domain that likely mediates protein-protein interactions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 232–331 of human NUDC, a region that may provide good epitope accessibility. When using this antibody for Western blot, a band around 38 kDa is anticipated; verify in your sample system.
• For ELISA applications, optimize coating or detection conditions by titrating the antibody to determine the optimal working concentration.
• Cross-reactivity with mouse and rat NUDC is expected based on sequence homology; however, validate performance in these species-specific systems.
• Consider using positive control cell lines with high NUDC expression, such as HeLa or HEK293, based on ubiquitous expression data.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, laboratories, and research institutes with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For targets like NUDC involved in cell cycle and neurogenesis, CamelBio can source or develop rare raw materials to accelerate your assay development. Our integrated technical services and consulting help streamline your path from concept to clinic.