Anti-NUDC Polyclonal Antibody for WB, ELISA - Q9Y266

Core Product Specifications and Parameters

Biological Background: NUDC Function and Localization

• NUDC (Nuclear distribution protein C homolog), also known as HNUDC, MNUDC, NPD011, is encoded by the gene NUDC (GeneID: 10726) in humans.
• It plays a role in neurogenesis and neuronal migration (By similarity).
• NUDC is necessary for correct formation of mitotic spindles and chromosome separation during mitosis. Related references: PMID:12679384 PMID:12852857 PMID:25789526
• NUDC is also necessary for cytokinesis and cell proliferation. Related references: PMID:12679384 PMID:12852857
• The protein localizes to the cytoplasm, cytoskeleton, spindle, midbody, and nucleus.
• It is ubiquitously expressed, with high levels in fetal liver, kidney, lung, and brain, and in adult pancreas, kidney, skeletal muscle, liver, lung, placenta, prostate, brain, and heart.
• NUDC undergoes acetylation and phosphorylation, and contains coiled-coil domains, suggesting roles in protein-protein interactions and regulation during the cell cycle.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal 1 – 331 amino acids of human NUDC, covering a significant portion of the protein; thus, the resulting polyclonal antibody may recognize multiple linear and conformational epitopes.
• For Western blot (WB), a predicted band around 38 kDa is expected; consider optimizing blocking and antibody dilution in the relevant sample type (e.g., HeLa, HEK293, or tissue lysates from human, mouse, or rat).
• When using ELISA, the recombinant immunogen can serve as a standard or coating antigen; however, assay conditions should be empirically determined.
• Due to the reported cross-reactivity with mouse and rat, this antibody is a candidate for studies in rodent models, but verify specificity via knockout controls or siRNA knockdown if possible.
• Given NUDC’s role in mitosis, synchronization of cell cultures may enhance detection in functional studies.

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CamelBio offers a streamlined sourcing experience for IVD developers and researchers, providing high-quality raw materials like this NUDC polyclonal antibody, along with complementary reagents for cell cycle and neurobiology studies. Our portfolio includes validated antibody pairs, bulk monoclonal and polyclonal antibodies, antigens, and ancillary reagents, all backed by technical support to accelerate your diagnostic assay development. Let CamelBio be your reliable partner from concept to clinic, delivering the raw materials you need with efficiency and expertise.