Anti-Nucleophosmin Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P06748

Core Product Specifications and Parameters

Biological Background: Nucleophosmin Function and Localization

• Nucleophosmin (NPM1/B23) is a multifunctional nucleolar phosphoprotein involved in ribosome biogenesis, centrosome duplication, and histone assembly.
• It acts as a chaperonin for core histones H3, H2B, and H4, facilitating nucleosome assembly.
• Binds single-stranded nucleic acids and associates with nucleolar ribonucleoprotein structures, likely aiding ribosome nuclear export.
• Regulates tumor suppressors p53/TP53 and ARF, and negatively regulates EIF2AK2/PKR to suppress apoptosis. Related references: PMID:22528486
• In complex with MYC, enhances transcription of MYC target genes. Related references: PMID:25956029
• Localizes primarily to the nucleolus, and also found in nucleoplasm, cytoplasm, and centrosome.
• Undergoes various post-translational modifications including phosphorylation (by PLK2 for centriole replication), acetylation, ADP-ribosylation, and ubiquitination; classified as a proto-oncogene with roles in chromosomal rearrangement.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a conserved N-terminal region (aa 9–122) of human NPM1, suggesting potential cross-reactivity with mouse and rat orthologs, as confirmed by the supplier.
• For WB, a band at approximately 33 kDa is expected; consider optimizing protein extraction and loading controls.
• IHC-P and IF/ICC may require antigen retrieval and permeabilization, respectively; validate in relevant positive control tissues.
• ELISA can be used for quantitative detection; ensure proper coating and detection conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-nucleophosmin monoclonal antibody supports research into ribosome biogenesis, centrosome dynamics, and tumor suppressor pathways. CamelBio also offers validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to accelerate your assay development.