Anti-Nucleolin (NCL) Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P19338

Core Product Specifications and Parameters

Biological Background: Nucleolin Function and Localization

• Nucleolin (NCL), also known as Protein C23, is the major nucleolar protein of growing eukaryotic cells.
• It plays a central role in ribosome biogenesis by associating with intranucleolar chromatin and pre-ribosomal particles, and is involved in pre-rRNA transcription and processing.
• Nucleolin induces chromatin decondensation through binding to histone H1, thereby regulating chromatin structure.
• It participates in transcriptional elongation and can bind both RNA and DNA, including specific telomeric repeat sequences.
• Nucleolin is predominantly localized in the nucleolus but can also be found in the nucleus and cytoplasm.
• Post-translational modifications include acetylation, phosphorylation, methylation, and ubiquitination, which regulate its diverse functions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 300-466 of human nucleolin; the resulting polyclonal antibody may recognize multiple epitopes within this region.
• For Western blot, consider using the predicted molecular weight of ~77 kDa as a reference; validate band specificity with appropriate controls.
• In IHC-P, antigen retrieval conditions and antibody dilution should be optimized for each tissue type; cross-reactivity with human and mouse enables broad applications.
• ELISA can be employed to assess antibody titer or specificity; ensure coating and detection protocols are standardized.
• Always include positive and negative controls to confirm antibody performance in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting. This anti-nucleolin antibody supports research into nucleolar biology, ribosome biogenesis, and chromatin regulation. In addition to validated antibody pairs and optimized monoclonal/polyclonal antibodies, CamelBio offers bulk ancillary reagents and rare target sourcing to streamline your workflow from concept to clinic.