Anti-Nucleolin Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P19338

Core Product Specifications and Parameters

Biological Background: Nucleolin Function and Localization

• Nucleolin (also known as Protein C23) is the major nucleolar protein in growing eukaryotic cells, playing a central role in ribosome biogenesis.
• It induces chromatin decondensation by binding to histone H1, thereby facilitating transcriptional processes and pre-rRNA transcription.
• Nucleolin participates in ribosomal assembly and may contribute to transcriptional elongation.
• It binds RNA motifs with 5’-UUAGGG-3’ repeats more tightly than the corresponding telomeric DNA repeats (5’-TTAGGG-3’).
• Subcellular localization is predominantly nucleolar and nuclear, with additional presence in the cytoplasm.
• Post-translational modifications include acetylation, methylation, phosphorylation, and ubiquitination, reflecting its dynamic regulatory roles.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the N-terminal region (aa 1-100) of human Nucleolin. Consider verifying epitope accessibility in your specific assay format.
• For Western blotting, use lysates from proliferating cells (e.g., HeLa) where nucleolin is highly expressed; an ~77 kDa band is expected under reducing conditions.
• In immunofluorescence (IF/ICC), a nucleolar staining pattern should be observed. Co-staining with a nucleolar marker (e.g., fibrillarin) is recommended to confirm localization.
• For IHC-P, optimize antigen retrieval and validate staining specificity using appropriate tissue controls.
• Adjust antibody dilution for each application and validate in the relevant sample system before routine use.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw-material partner, CamelBio streamlines the procurement of critical reagents from concept to clinic. This anti-Nucleolin monoclonal antibody supports investigations into chromatin dynamics and ribosome biogenesis, and CamelBio can supply matched antibody pairs, bulk ancillary reagents, and assist in sourcing rare-target raw materials to accelerate your diagnostic or research applications.