Anti-Nucleobindin-1 (NUCB1) Rabbit Polyclonal Antibody for WB and ELISA - Q02818

Core Product Specifications and Parameters

Biological Background: NUCB1 Function and Localization

• Nucleobindin-1 (NUCB1), also designated CALNUC, is a major calcium-binding protein predominantly localized in the Golgi apparatus.
• It is implicated in maintaining calcium homeostasis within the cis-Golgi network and functions as a non-receptor guanine nucleotide exchange factor for G proteins, thereby modulating signal transduction.
• Subcellularly, NUCB1 is anchored to the cis-Golgi membrane but is also detected in the cytoplasm and as a secreted protein, suggesting multiple functional compartments.
• Tissue distribution studies reveal expression in fetal and adult heart, lung, liver, kidney, and brain, with additional adult expression in skeletal muscle, placenta, and pancreas.
• Structurally, it features a signal peptide, coiled-coil domains, DNA-binding motifs, and EF-hand calcium-binding repeats; post-translational modifications include glycosylation and phosphorylation.
• Key annotated keywords include calcium, metal-binding, glycoprotein, phosphoprotein, DNA-binding, and Golgi apparatus, reflecting its diverse biochemical interactions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (residues 302–461) of human NUCB1, which may provide specificity for the target in WB and ELISA applications.
• For Western blot, consider using reduced and non-reduced conditions to assess reactivity, and validate band identity with appropriate controls at the predicted molecular weight (~54 kDa).
• Since cross-reactivity is reported only for human, experimental validation should be performed in human-derived cell lines or tissue lysates.
• When using ELISA, titration of the antibody and antigen coating concentrations is recommended to optimize signal-to-noise ratios.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research teams with premium IVD raw materials, including validated primary antibodies like this anti-NUCB1 polyclonal. Beyond single reagents, we support your pipeline with matched antibody pairs, optimized monoclonal alternatives, bulk ancillary reagents, and custom sourcing for rare targets. Whether you are advancing calcium signaling or Golgi biology projects, CamelBio streamlines procurement from concept to clinic.